SOX2-OT/SOX2 axis regulates lung cancer H520 cell migration via Gli1-mediated epithelial-mesenchymal transition.
10.12122/j.issn.1673-4254.2022.10.01
- Author:
Hongliang DONG
1
;
Lili ZENG
1
;
Yan WU
1
;
Shuang MIAO
1
;
Na NI
1
;
Naiguo LIU
1
;
Weiwei CHEN
1
;
Jing DU
1
Author Information
1. Medical Research Center, Binzhou Medical University Hospital, Binzhou 256600, China.
- Publication Type:Journal Article
- Keywords:
Gli1;
SOX2;
SOX2-OT;
epithelial-mesenchymal transition;
lung squamous cell carcinoma
- MeSH:
Humans;
Epithelial-Mesenchymal Transition/genetics*;
Zinc Finger Protein GLI1/metabolism*;
Cell Line, Tumor;
Cell Movement/genetics*;
Lung Neoplasms/genetics*;
MicroRNAs/metabolism*;
Gene Expression Regulation, Neoplastic;
Cell Proliferation/genetics*;
Neoplasm Invasiveness/genetics*;
SOXB1 Transcription Factors/metabolism*
- From:
Journal of Southern Medical University
2022;42(10):1431-1439
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the regulatory role of SOX2-OT in migration of lung squamous cell carcinoma H520 cells and the underlying mechanisms.
METHODS:Wound- healing and Transwell migration assays were performed to examine the changes in migration and invasion capacity of lung squamous cell line H520, which expressed higher levels of SOX2-OT than other lung cancer cell lines, following RNA interference-mediated SOX2-OT knockdown. The transcription levels of epithelial-mesenchymal transition (EMT)-related components was detected by qRT-PCR and immunoblotting. Gli1 gain-of-function analysis was performed in H520 cells with SOX2-OT knockdown and the changes in EMT phenotype of the cells were examined. miR-200c mimic and inhibitor were used to analyze the mechanism by which SOX2-OT positively regulates Gli1 and the mediating role of SOX2.
RESULTS:SOX2-OT knockdown significantly lowered the invasiveness and migration capacity of H520 cells and caused changes in EMT phenotype of the cells. Overexpression of Gli1, which was positively regulated by SOX2-OT, reversed the inhibitory effect of SOX2-OT knockdown on migration of H520 cells. Transfection of the cells with miR-200c inhibitor effectively reversed SOX2-OT knockdown-induced down-regulation of SOX2.
CONCLUSION:The SOX2-OT/SOX2 axis positively regulates migration of lung squamous H520 cells via Gli1-mediated EMT.
