Effect of baotaiyin on IL-23 /Th17 immune inflammatory axis in mouse model of spontaneous abortion.
10.11817/j.issn.1672-7347.2022.220117
- Author:
Xingxiu ZHAN
1
;
Lijuan JIANG
2
;
Hongping NIU
3
;
Lijuan YANG
3
;
Qianqian WAN
4
;
Yanping QIAN
4
Author Information
1. First Clinical College of Nanjing University of Chinese Medicine, Nanjing 210046. 447220621@qq.com.
2. Department of Gynecology, Affiliated Hospital of Yunnan University of Chinese Medicine, Kunming 650000. jianglijuan825@163.com.
3. Department of Gynecology, Yunnan University of Chinese Medicine, Kunming 650500, China.
4. Department of Gynecology, Affiliated Hospital of Yunnan University of Chinese Medicine, Kunming 650000.
- Publication Type:Journal Article
- Keywords:
IL-23/Th17;
Th17/Treg;
baotaiyin;
spontaneous abortion;
traditional Chinese medicine
- MeSH:
Mice;
Animals;
Female;
Humans;
Pregnancy;
Interleukin-23;
Interleukin-17/genetics*;
Interleukin-10;
Abortion, Habitual;
Transforming Growth Factor beta/genetics*
- From:
Journal of Central South University(Medical Sciences)
2022;47(11):1532-1539
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:The mechanism for traditional Chinese medicine in treating of recurrent spontaneous abortion is not clear. This study aims to explore the mechanism of baotaiyin in the treatment of recurrent abortion by regulating the immune inflammatory axis of interleukin (IL)-23/helper T cell (Th)17.
METHODS:Spontaneous abortion model mice were randomly divided into a model group, 3 dose (low, medium, and high) groups of baotaiyin, with 10 mice in each group. After 14 days of medication, the levels of IL-17, IL-23, IL-10, and TGF-β in serum were detected with enzyme-linked immunosorbent assay. The proportion of Th17 and regulatory T cells (Treg) cells in spleen lymphocytes was tested with flow cytometry. The expressions of (retinoid-related orphan receptor γt, ROR-γt) and forkhead box P3 (FOXP3) mRNA in decidua tissues was detected with RT-PCR. Embryo absorption rate was counted.
RESULTS:Compared with the model group, the absorption rate of embryo and Th17/Treg cell ratio in baotaiyin medium- and high-dose groups were decreased significantly (all P<0.05); the levels of IL-17 and IL-23 in serum were decreased (both P<0.05), while the levels of TGF-β and IL-10 in baotaiyin medium- and high-dose groups were increased (P<0.05, P<0.01, respectively); the expression of ROR-γt mRNA was decreased and the expression of FOXP3 mRNA was increased (all P<0.01) in decidua tissues of baotaiyin medium- and high-dose groups.
CONCLUSIONS:Baotaiyin inhibits the positive feedback cycle of IL-23/Th17 immune inflammatory axis, which regulates Th17/Treg cell balance, mediates the maternal and fetal immune tolerance, and prevents the recurrent abortion.
