3D Microfluidic System for Evaluating Inhibitory Effect of Chinese Herbal Medicine Oldenlandia diffusa on Human Malignant Glioma Invasion Combined with Network Pharmacology Analysis.
10.1007/s11655-021-3726-1
- Author:
Chun-Hui HAN
1
;
Jing-Yun MA
1
;
Wei ZOU
2
;
Jia-Lin QU
3
;
Yang DU
4
;
Na LI
1
;
Yong LIU
4
;
Guo JIN
2
;
Ai-Jing LENG
4
;
Jing LIU
5
Author Information
1. Stem Cells Clinical Research Institution, the First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Liaoning Province, China.
2. Dalian Innovation Institute of Stem Cell and Precision Medicine, Dalian, 116023, Liaoning Province, China.
3. Integrated Chinese and Western Medicine Laboratory, the First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Liaoning Province, China.
4. Department of Traditional Chinese Medicine, the First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Liaoning Province, China.
5. Stem Cells Clinical Research Institution, the First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Liaoning Province, China. liujing@dmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
Oldenlandia diffusa;
invasion;
malignant glioma;
microfluidic chip
- From:
Chinese journal of integrative medicine
2023;29(1):52-60
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the anti-invasion efficacy of the ethanol extract of Oldenlandia diffusa Will. (EEOD) on a three-dimensional (3D) human malignant glioma (MG) cell invasion and perfusion model based on microfluidic chip culture and the possible mechanism of action of Oldenlandia diffusa Will. (OD).
METHODS:The comprehensive pharmacodynamic analysis method in this study was based on microfluidic chip 3D cell perfusion culture technology, and the action mechanism of Chinese medicine (CM) on human MG cells was investigated through network pharmacology analysis. First, the components of EEOD were analyzed by ultraperformance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Then, cell viability and apoptosis were assessed to determine the optimum concentration of EEOD for invasion experiments, and two-dimensional (2D) migration and invasion abilities of U87 and U251 MG cells were evaluated using scratch wound and Transwell assays. The possible mechanism underlying the effects of EEOD on glioma was analyzed through a network pharmacology approach.
RESULTS:Thirty-five compounds of EEOD were detected by UPLC-Q-TOF/MS. EEOD suppressed the viability of MG cells, promoted their apoptosis, and inhibited their migratory and invasive potentials (all P<0.05). Network pharmacology analysis showed that OD inhibited the invasion of MG cells by directly regulating MAPK and Wnt pathways through MAPK, EGFR, MYC, GSK3B, and other targets. The anti-invasion effect of OD was also found to be related to the indirect regulation of microtubule cytoskeleton organization.
CONCLUSIONS:]EEOD could inhibit the invasion of human MG cells, and the anti-invasion mechanism of OD might be regulating MAPK and Wnt signaling pathways and microtubule cytoskeleton organization.
