Acupuncture and Moxibustion Inhibited Intestinal Epithelial-Mesenchymal Transition in Patients with Crohn's Disease Induced by TGF- β 1/Smad3/Snail Pathway: A Clinical Trial Study.
10.1007/s11655-022-2888-1
- Author:
Sen GUO
1
;
Jing ZHOU
2
;
Liang ZHANG
1
;
Chun-Hui BAO
3
;
Ji-Meng ZHAO
3
;
Yan-Ling GAO
1
;
Pin WU
1
;
Zhi-Jun WENG
3
;
Yin SHI
4
Author Information
1. Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
2. Department of Acupuncture and Moxibustion, Shanghai Shanghai Traditional Chinese Medicine Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200082, China.
3. Department of Acupuncture and Moxibustion, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China.
4. Department of Acupuncture and Moxibustion, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China. flysy0636@163.com.
- Publication Type:Randomized Controlled Trial
- Keywords:
Crohn’s disease;
acupuncture;
intestinal epithelial-mesenchymal transition;
moxibustion;
transforming growth factor β 1/Smad3/Snail pathway
- MeSH:
Acupuncture Therapy;
Cadherins/metabolism*;
Crohn Disease/therapy*;
Epithelial-Mesenchymal Transition;
Fibronectins/metabolism*;
Humans;
Moxibustion;
Smad3 Protein/metabolism*;
Snail Family Transcription Factors/metabolism*;
Transforming Growth Factor beta1/metabolism*
- From:
Chinese journal of integrative medicine
2022;28(9):823-832
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore whether acupuncture combined with moxibustion could inhibit epithelialmesenchymal transition in Crohn's disease by affecting the transforming growth factor β 1 (TGF- β 1)/Smad3/Snail pathway.
METHODS:Sixty-three patients with Crohn's disease were randomly divided into an observation group (31 cases) receiving moxibustion at 43 °C combined with acupuncture, and a control group (32 cases) receiving moxibustion at 37 °C combined with sham acupuncture using a random number table. Patients were treated for 12 weeks. Crohn's Disease Activity Index (CDAI) was used to evaluate disease activity. Hematoxylin-eosin staining and transmission electron microscopy were utilized to observe the morphological and ultrastructural changes. Immunohistochemistry was used to detect the expression of transforming growth factor β 1 (TGF-β 1), T β R1, T β R2, Smad3, Snail, E-cadherin and fibronectin in intestinal mucosal tissues.
RESULTS:The decrease of the CDAI score, morphological and ultrastructural changes were more significant in observation group. The expression levels of TGF- β 1, Tβ R2, Smad3, and Snail in the observation group were significantly lower than those before the treatment (P<0.05 or P<0.01). After treatment, the expression levels of TGF-β 1, TβR2, and Snail in the observation group were significantly lower than those in the control group (all P<0.05); compared with the control group, the expression of fibronectin in the observation group was significantly decreased, and the expression of E-cadherin was significantly increased (all P<0.05).
CONCLUSIONS:Moxibustion at 43 °C combined with acupuncture may suppress TGF-β 1/Smad3/Snail pathway-mediated epithelial-mesenchymal transition of intestinal epithelial cells in Crohn's disease patients by inhibiting the expression levels of TGF-β 1, Tβ R2, Smad3, and Snail. (Registration No. ChiCTR-IIR-16007751).
