Seabuckthorn Berries Extract Attenuates Pulmonary Vascular Hyperpermeability in Lipopolysaccharide-Induced Acute Lung Injury in Mice.
10.1007/s11655-021-3346-1
- Author:
Lei-Lei DU
1
;
Ying LIU
2
;
Li WAN
3
;
Chu CHEN
4
;
Gang FAN
5
Author Information
1. School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
2. School of Basic Medicine, Chengdu University, Chengdu, 610106, China.
3. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
4. Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences, Chengdu, 610041, China.
5. School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. fangang1111@163.com.
- Publication Type:Journal Article
- Keywords:
acute lung injury;
neutrophil;
nuclear factor-kappa B;
pulmonary vascular hyperpermeability;
seabuckthorn berries extract
- MeSH:
Animals;
Mice;
Acute Lung Injury/drug therapy*;
Fruit/chemistry*;
Hippophae/chemistry*;
Intercellular Adhesion Molecule-1/metabolism*;
Interleukin-6/metabolism*;
Lipopolysaccharides;
Lung/pathology*;
NF-kappa B/metabolism*;
Tumor Necrosis Factor-alpha/metabolism*;
Plant Extracts/therapeutic use*
- From:
Chinese journal of integrative medicine
2022;28(12):1081-1087
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effect of seabuckthorn berries extract (SBE) on pulmonary vascular hyperpermeability in the mice model of acute lung injury (ALI) induced by lipopolysaccharide (LPS).
METHODS:Sixty Kunming mice were allocated into 6 groups by a random number table, including control, LPS, dexamethasone (Dex, 1 mg/kg), and 120, 240 and 480 mg/kg SBE groups, 10 mice in each group. Except the control group, mice were pre-treated with Dex and SBE, respectively, for 7 days before LPS was intraperitoneally injected to induce ALI. Pulmonary vascular hyperpermeability was evaluated by histopathologic observation and transvascular leakage determination. Tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) levels in serum were measured using enzyme-linked immunosorbent assay. The expression of nuclear factor-kappa B (NF-κB) p65 in lung cells was determined by immunofluorescence analysis. The contents of cytoplasmic inhibitor of nuclear factor-κB kinase (IKK) and nuclear p65, as well as downstream proteins of E-selectin (CD62E) and intercellular adhesion molecule-1 (ICAM-1), were determined using Western blot analysis.
RESULTS:Histopathological observation confirmed SBE treatment alleviated morphological lesion induced by LPS. Compared with the LPS group, 480 mg/kg SBE significantly decreased the water content of lung, Evans blue accumulation in lung tissue, and protein concentration and neutrophils count in bronchoalveolar lavage fluid (P<0.01); moreover, 480 mg/kg SBE significantly suppressed release of TNF-α and IL-6, and down-regulated expressions of IKK, nuclear p65, ICAM-1 and CD62E (P<0.01).
CONCLUSION:SBE maintained alveolar-capillary barrier integrity under endotoxin challenge in mice by suppressing the key factors in the pathogenesis of ALI.
