Sijunzi Decoction Inhibits Stemness by Suppressing β-Catenin Transcriptional Activity in Gastric Cancer Cells.
10.1007/s11655-021-3314-9
- Author:
Yue-Jun LI
1
;
Lin-Li LIAO
2
;
Pei LIU
2
;
Ping TANG
1
;
Hong WANG
1
;
Qing-Hua PENG
3
Author Information
1. Department of Oncology, the Third Affiliated Hospital of Hunan University of Chinese Medicine, Zhuzhou, 412000, Hunan Province, China.
2. Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China.
3. Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China. pqh410007@126.com.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
Sijunzi Decoction;
cancer stem cells;
gastric cancer;
β-catenin
- MeSH:
Cell Line, Tumor;
DNA/metabolism*;
Drugs, Chinese Herbal/pharmacology*;
Humans;
Neoplastic Stem Cells/metabolism*;
Stomach Neoplasms/genetics*;
beta Catenin/metabolism*
- From:
Chinese journal of integrative medicine
2022;28(8):702-710
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate a previously uncharacterized function of Sijunzi Decoction (SJZD) in inhibition of gastric cancer stem cells (GCSCs).
METHODS:MKN74 and MKN45, two CD44 positive gastric cancer cell lines with stem cell properties were used. The cells were divided into 2 groups. Treatment group was treated with SJZD (1-5 mg/mL) for indicated time (48 h-14 days). The control group was treated with equal volume of phosphate buffered saline. Cell Counting Assay Kit-8 were used to measure cell viability. Spheroid colony formation and GCSCs marker expression were performed to determine GCSCs stemness. Cell fractionation and chromatin immunoprecipitation assays were used to assess the distribution and DNA-binding activity of β-catenin after SJZD treatment, respectively.
RESULTS:SJZD treatment repressed cell growth and induced apoptosis in MKN74 and MKN45 cell lines (P<0.05). Moreover, SJZD dramatically inhibited formation of spheroid colony and expression of GCSC markers in GC cells (P<0.05). Mechanistically, SJZD reduced nuclear accumulation and DNA binding activity of β-catenin (P<0.05), the key regulator for maintaining CSC stemness.
CONCLUSION:SJZD inhibits GCSCs by attenuating the transcriptional activity of β-catenin.
