Effect of Curcumol on NOD-Like Receptor Thermoprotein Domain 3 Inflammasomes in Liver Fibrosis of Mice.
10.1007/s11655-021-3310-0
- Author:
Yang ZHENG
1
;
Lei WANG
1
;
Jia-Hui WANG
1
;
Lu-Lu LIU
2
;
Tie-Jian ZHAO
3
Author Information
1. Department of Medicine, Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning, 530021, China.
2. Department of Teaching, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, 530022, China.
3. Department of Physiology, College of Basic Medicine, Guangxi University of Chinese Medicine, Nanning, 530021, China. 13014989836@163.com.
- Publication Type:Journal Article
- Keywords:
NOD-like receptor thermoprotein domain 3;
curcumol;
inflammatory body;
liver fibrosis
- MeSH:
Animals;
Mice;
Inflammasomes/metabolism*;
Interleukin-1beta/metabolism*;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
NLR Proteins;
Caspase 1;
Tumor Necrosis Factor-alpha;
Carbon Tetrachloride;
Hematoxylin;
Saline Solution;
Eosine Yellowish-(YS);
Peanut Oil;
Liver Cirrhosis/drug therapy*;
RNA, Messenger/genetics*;
Collagen;
Transforming Growth Factor beta
- From:
Chinese journal of integrative medicine
2022;28(11):992-999
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effect of curcumol on NOD-like receptor thermoprotein domain 3 (NLRP3) inflammasomes, and analyze the mechanism underlying curcumol against liver fibrosis.
METHODS:Thirty Kunming mice were divided into a control group, a model group and a curcumol group according to a random number table, 10 mice in each group. Mice were intraperitoneally injected with 40% carbon tetrachloride (CCl4:peanut oil, 2:3 preparation) at 5 mL/kg for 6 weeks, twice a week, for developing a liver fibrosis model. The mice in the control group were given the same amount of peanut oil twice a week for 6 weeks. The mice in the curcumol group were given curcumol (30 mL/kg) intragastrically, and the mice in the model and control groups were given the same amount of normal saline once a day for 6 weeks. Changes in liver structure were observed by hematoxylin and eosin (HE) and Masson staining. Liver function, liver fiber indices, and the expression of interleukin (IL)-10 and tumor necrosis factor-α (TNF-α) levels were determined by automatic biochemical analyzer and enzyme linked immunosorbent assay kit. Immunoblotting and reverse transcription-quantitative PCR (RT-qPCR) were performed to detect the expression of NLRP3 inflammasome-related molecules, TGF-β and collagen.
RESULTS:HE and Masson staining results showed that the hepatocytes of the model group were arranged irregularly with pseudo-lobular structure and a large amount of collagen deposition. The mice in the curcumol group had a significant decrease in liver function and liver fibers indices compared with the model group (P<0.05); RT-qPCR and Western blotting results reveal that, in the curcumol group, the mRNA and protein expression levels of NLRP3, IL-1 β, Caspase 1 and gasdermin D decreased significantly compared with the model group (P<0.05); immunohistochemical results showed that in the curcumol group, the protein expression levels of NLRP3 and IL-1 β decreased significantly compared with the model group (P<0.05).
CONCLUSION:A potential anti-liver fibrosis mechanism of curcumol may be associated with the inhibition of NLRP3 inflammasomes and decreasing the downstream inflammatory response.
