Study on Characterization and Content Determination Method of Silver in Chitosan Antibacterial Gel.
10.3969/j.issn.1671-7104.2022.05.017
- Author:
Jun PENG
1
;
Shuhan WANG
1
;
Wenliang LIU
1
;
Haoqi KE
1
;
Xiaofeng GAO
1
Author Information
1. Shenzhen Institute for Drug Control (Shenzhen Testing Center of Medical Devices), Shenzhen, 518057.
- Publication Type:Journal Article
- Keywords:
antibacterial gel;
atomic absorption spectrophotometry;
characterize;
content;
silver
- MeSH:
Anti-Bacterial Agents/pharmacology*;
Chitosan/chemistry*;
Microwaves;
Particle Size;
Silver
- From:Chinese Journal of Medical Instrumentation
2022;46(5):560-564
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To characterize the silver in chitosan antibacterial gel, and to establish a method for the determination of silver content in samples.
METHODS:The silver in the samples was analysed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and single particle inductively coupled plasma mass spectrometry (SP-ICP-MS). Microwave digestion was adopted to digest the chitosan antibacterial gel, and then the content of silver was determined by flame atomic absorption spectrometry.
RESULTS:The analysises showed that the particle size of silver in chitosan antibacterial gel was about 150~ 200 nm. The silver showed good linearity in the concentration range of 25~250 μg/L (y=0.000 35x+0.001 7, r=0.999 9). The recovery rate (n=9) was 98.5%.
CONCLUSIONS:SEM, EDS and SP-ICP-MS can be used for the characterization of silver particles in chitosan antibacterial gel. Microwave digestion-flame atomic absorption spectrophotometry method is simple, practicable, high precision and high quantitative accuracy, which is suitable for the quantitative analysis of silver in chitosan antibacterial gel.
