Factors Affecting the Preservation of Human Peripheral Blood Mononuclear Cells at 4 ℃.
10.19746/j.cnki.issn.1009-2137.2022.06.040
- Author:
Xuan ZHANG
1
;
Lei WANG
2
;
Dong-Dong LI
2
;
Shao-Duo YAN
2
;
Kun LIU
1
;
Yi-Zhe ZHENG
1
;
Feng-Ying QI
1
;
Zi-Xin HE
1
;
Yan-Hong LI
2
;
Bo WANG
2
;
Qiu-Xia FU
3
;
Jun LIANG
4
Author Information
1. Department of Light Industry Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China,Institute of Health Services and Blood, Academy of Military Medical Sciences, Beijing 100039, China.
2. Institute of Health Services and Blood, Academy of Military Medical Sciences, Beijing 100039, China.
3. Institute of Health Services and Blood, Academy of Military Medical Sciences, Beijing 100039, China,E-mail：tiger_qhd@sina.com.
4. Department of Light Industry Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China,E-mail：jliang1118@yeah.net.
- Publication Type:Journal Article
- Keywords:
cell preservation at 4 ℃;
glucose;
human peripheral blood mononuclear cells;
human serum albumin
- MeSH:
Humans;
Leukocytes, Mononuclear
- From:
Journal of Experimental Hematology
2022;30(6):1881-1886
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the preservation effect and related influencing factors of human peripheral blood mononuclear cells under serum-free condition at 4 ℃.
METHODS:Human peripheral blood mononuclear cells were isolated by density gradient centrifugation, and stored at 4 ℃ under different cell concentrations, supplemented with human serum albumin, and glucose. The cell viability, total cell number, viable cell number and cell phenotype were detected during preservation of 72 h.
RESULTS:With the prolongation of storage time, the number of human peripheral blood mononuclear cells gradually decreased(r=0.982). Compared with the cell concentration of (5-6)×106 cells/ml, the cell number decreased more slowly when the cell storage concentration was (1-2)×106 cells/ml; Adding human serum albumin and glucose can effectively improve the survival rate of human peripheral blood mononuclear cells, among which 2% human serum albumin has a better preservation effect; Compared with the blank control group, the analysis results of cell subsets showed that the downward trends of NK cells and T cells were significantly slowed after adding albumin and glucose.
CONCLUSION:The cell density of (1-2)×106/ml and 2% human serum albumin are more suitable for the preservation of PBMC, and 5% glucose can improve the preservation effect of human peripheral blood mononuclear cells at 4 ℃.
