Effects of Different Treatment Methods on the Contents of Related Growth Factors Released by Platelet Rich Plasma.
10.19746/j.cnki.issn.1009-2137.2022.06.032
- Author:
Shu-Jun WANG
1
;
Guang-Chao ZHAO
2
;
Kai-Yun LUO
2
;
Ying DU
3
;
Wei WANG
2
;
Qing QI
2
;
Jian-Feng LUAN
4
Author Information
1. Department of Blood Transfusion Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China,Southeast University School of Medicine, Nanjing 210009, Jiangsu Province, China.
2. Department of Blood Transfusion Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China.
3. Southeast University School of Medicine, Nanjing 210009, Jiangsu Province, China.
4. Department of Blood Transfusion Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China,E-mail: luanjf1003@126.com.
- Publication Type:Journal Article
- Keywords:
growth factor;
platelet rich plasma;
treatment method
- MeSH:
Humans;
Transforming Growth Factor beta1;
Vascular Endothelial Growth Factor A;
Calcium Gluconate;
Calcium;
Calcium Chloride;
Becaplermin;
Platelet-Rich Plasma
- From:
Journal of Experimental Hematology
2022;30(6):1834-1838
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the effect of sonication, repeated freeze-thaw cycles, calcium salt solution and their combination on the content of related growth factors (GFs) released by platelet rich plasma (PRP).
METHODS:Twenty PRPs from healthy blood donors were divided into 9 groups, including sonication group, freeze-thaw group, calcium gluconate group, calcium chloride group, sonication + calcium gluconate group, sonication + calcium chloride group, freeze-thaw + calcium gluconate group, freeze-thaw + calcium chloride group, and sonication + freeze-thaw group. After PRP activated by above 9 methods, the content of transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor-BB (PDGF-BB) were detected by ELISA.
RESULTS:The platelet concentration of the samples was (966.7±202.6)×109/L. The content of TGF-β1 in sonication + freeze-thaw group was the highest, while the lowest was in freeze-thaw group. The content of VEGF in freeze-thaw + calcium chloride group was the highest, while the lowest was in calcium gluconate group. The content of PDGF-BB in sonication + freeze-thaw group was the highest, while the lowest was in calcium gluconate group. There was no significant differences in the three GFs between calcium gluconate group and calcium chloride group.
CONCLUSION:Among the 9 activated methods of PRP, there is no difference between two calcium salt solutions. And the combination of repeated freeze-thaw cycles and sonication may be the best treatment method to promote PRP to release GFs, while calcium gluconate is the weakest way.
