Bacteriostasis of Platelet-Rich Plasma and Its Derivatives in Vitro and Its Relationship with PF4.
10.19746/j.cnki.issn.1009-2137.2022.06.031
- Author:
Ming-Zhu SONG
1
;
Mei-Mei LI
1
;
Xiao-Xia NIE
1
;
Jia LIU
1
;
Juan CAI
2
Author Information
1. Department of Blood Transfusion, Luan People's Hospital Affiliated to Anhui Medical University, Luan 237005, Anhui Province, China.
2. Department of Blood Transfusion, Luan People's Hospital Affiliated to Anhui Medical University, Luan 237005, Anhui Province, China,E-mail: caijuan775@163.com.
- Publication Type:Journal Article
- Keywords:
escherichia coli;
platelet factor 4;
platelet-rich plasma
- MeSH:
Adult;
Humans;
Middle Aged;
Escherichia coli;
Platelet Factor 4;
Platelet-Rich Plasma
- From:
Journal of Experimental Hematology
2022;30(6):1829-1833
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the bacteriostatic effect of platelet-rich plasma (PRP) and its derivative platelet gel (PG) supernatant on Escherichia coli in vitro and its relationship with platelet factor 4 (PF4).
METHODS:Apheresis platelets donated by healthy volunteers were obtained from the Blood station of Lu an Blood Center as the source of PRP. The counts of platelet, white blood cell and red blood cell in PRP and its derivative PG supernatant were detected by automatic hematology analyzer. Bacterial growth of PRP and PG supernatants co-cultured with bacteria for different time was observed by plate coating culture method, and the contents of PF4 in PRP and PG supernatants were detected by ELISA.
RESULTS:Apheresis platelets were collected from 28 healthy volunteers with a median age of 33 (21-56) years old. PRP can inhibit the growth of escherichia coli, but there were individual differences in antibacterial effect within 24 hours. PRP of 13 healthy volunteers had strong antibacterial effect at 24 hours, 7 cases had weak antibacterial effect at 24 hours, and 8 cases had no antibacterial effect at 24 hours. PG supernatant showed no significant individual difference, and all of them had bacteriostatic effect within 12 hours, but no bacteriostatic effect after 12 hours. There was no statistical difference in the bacteriostatic effect of PRP at 24 hours between healthy volunteers aged ≤30 years and >30 years (P>0.05), and there was no statistical difference between the white blood cell count ≤0.1×109/L and (0.1-1) ×109/L groups (P>0.05). There was significant difference in the bacteriostatic effect of PRP between the two groups with platelet content ≤1 000×109/L and >1 000×109/L (P<0.05). The platelet count in PRP was higher than that in PG supernatant ［(911.57±160.52) ×109/L vs 0］. The PF4 level in PRP was higher than that in PG supernatant (23623.34±9822.14 vs 6664.74±4065.83, P<0.05).
CONCLUSION:Both PRP and PG supernatant have antibacterial effects in Escherichia coli. The bacteriostatic effect of PRP was better than that of PG supernatant, and the platelet and PF4 contents in PRP were higher than those in PG supernatant, suggesting that the platelet and PF4 levels play an important role in bacteriostasis.
