Expression Levels of MiR-451 in Erythroid Differentiation and Its Correlation with Hematological Diseases.
10.19746/j.cnki.issn.1009-2137.2022.06.028
- Author:
Ling LING
1
;
Fang-Fang WANG
2
;
Shu-Ting ZHOU
1
;
Lei YANG
1
;
Fan YANG
1
;
Lan YANG
1
;
Duo-Nan YU
3
Author Information
1. Yangzhou University School of Medicine/Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, Yangzhou 225009, Jiangsu Province, China.
2. Clinical Medical College, Yangzhou University/Northern Jiangsu People's Hospital, Yangzhou 225001, Jiangsu Province, China.
3. Yangzhou University School of Medicine/Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, Yangzhou 225009, Jiangsu Province, China,E-mail: dnyu@yzu.edu.cn.
- Publication Type:Journal Article
- Keywords:
correlation;
erythroid differentiation;
hematological diseases;
microRNA-451
- MeSH:
Mice;
Animals;
beta-Thalassemia/genetics*;
Cell Differentiation;
MicroRNAs/genetics*
- From:
Journal of Experimental Hematology
2022;30(6):1810-1816
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of miR-451 during erythroid differentiation and its correlation with hematological diseases.
METHODS:The expression of miR-451 in erythroid differentiation of mouse hematopoietic stem cells (derived from fetal liver) was analyzed by cell culture, flow cytometry, magnetic bead sorting and qRT-PCR. The expression of miR-451 during erythroid differentiation of mouse erythroid leukemia cells (MEL) was analyzed by cell culture and qRT-PCR. The expression of miR-451 in peripheral blood of mice was detected by qRT-PCR, and the expression of miR-451 in fetal liver (14.5 days) was analyzed by microarray. The nucleated erythroid cells from bone marrow of wild type (WT) mice and β-thalassemia (β-thal) mice were sorted by flow cytometry, and the levels of miR-451 and erythroid genes were detected by qRT-PCR. The expression of miR-451 in peripheral blood of patients with clinical hematological diseases was detected by qRT-PCR.
RESULTS:During the differentiation of mouse hematopoietic stem cells (derived from fetal liver) and MEL cells, the expression levels of miR-451 increased gradually. Compared with WT mice, the expression levels of miR-451 in peripheral blood, 14.5-day fetal liver cells and nucleated erythroid cells (sorted from bone marrow) of β-thal mice were significantly increased(P<0.05). Many erythroid differentiation genes in nucleated erythroid cells (sorted from bone marrow) of β-thal mice decreased. Compared with healthy controls, the expression levels of miR-451 was increased in peripheral blood of patients with β-thalassemia and iron deficiency anemia, while the expression levels of miR-451 was decreased in patients with aplastic anemia and myelodysplastic syndrome.
CONCLUSION:During erythroid differentiation, the expression levels of miR-451 increases gradually. In hematological diseases, the expression levels of miR-451 is different from that of normal controls, which is expected to become an auxiliary diagnostic index for clinical hematological diseases.
