The Mechanism of Resveratrol on Acute T-Lymphocyte Leukemia through IL-7-Mediated JAK / STAT Signaling Pathway.
10.19746/j.cnki.issn.1009-2137.2022.06.014
- Author:
Min SHI
1
;
Pei-Ying KANG
1
;
Yi-Yao LI
1
;
Shao-Hua WANG
1
;
Yuan-Yuan ZHANG
1
;
Wen-Jing WANG
1
;
Yong-Jun LI
2
Author Information
1. Department of Clinical Laboratory, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
2. Department of Clinical Laboratory, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China,E-mail: lyjj221@sina.com.
- Publication Type:Journal Article
- Keywords:
IL-7;
JAK/STAT signaling pathway;
Pim1;
T-cell acute lymphoblastic leukemia;
resveratrol
- MeSH:
Female;
Mice;
Animals;
Mice, Inbred C57BL;
Resveratrol;
Signal Transduction;
Interleukin-7;
Janus Kinases;
STAT Transcription Factors;
RNA, Messenger;
T-Lymphocytes;
Leukemia
- From:
Journal of Experimental Hematology
2022;30(6):1715-1723
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the roles and relative mechanism of resveratrol against T-ALL through detecting the signaling molecules in IL-7 and JAK/STAT pathway.
METHODS:In vitro experiments, Molt4 cells were divided into 3 groups, including the control group, the DMSO group and resveratrol-treated group (Res group). The control group cells without any treatment, the DMSO group cells treated with 0.05% DMSO for 48 hours, the Res group cells treated with 200 μmol/L resveratrol for 48 hours. In vivo experiments, female C57BL/6J mice (6-8 weeks) were randomly divided into the control group, the T-ALL model group (T-ALL group), and Res treatment group (Res group). The control group mice treated with 0.05% DMSO by intragastric treatment, the T-ALL group mice treated with 0.05% DMSO by intragastric treatment, and the Res group mice treated with 10 mg/ml resveratrol. Expression of IL-7, IL-7R and Pim1 mRNA in the cells and mice spleen tissues were detected by RT-qPCR. Cell proliferation ability was detected by CCK-8. The expression of JAK1, JAK3, STAT5, phosphorylated JAK1 (p-JAK1), phosphorylated JAK3 (p-JAK3), phosphorylated STAT5 (p-STAT5) and Pim1 were detected by Western blot. ELISA was used to detect the IL-7 and IL-7R in the cells and mice serum of each groups.
RESULTS:Resveratrol could inhibit the proliferation ability of Molt4 cells, decrease the relative levels of p-JAK1, p-JAK3, p-STAT5, Pim1 protein, and the expression levels of Pim1, IL-7 and IL-7R mRNA in cells and mice spleens, reduce the IL-7 and IL-7R in Molt4 cells and mice serum.
CONCLUSION:Resveratrol may inhibite IL-7-medicated JAK/STAT signaling pathway to reduce the expression of target protein Pim1 to further exert its anti-T-ALL effects.
