Detection of Fusion Gene and Prognosis Analysis in Children with Acute Lymphoblastic Leukemia of Different Immunophenotypes.
10.19746/j.cnki.issn.1009-2137.2022.06.008
- Author:
Chun-Yan LIU
1
;
Yan-Sha PAN
2
;
Hong YANG
2
;
Xiao HU
2
;
Hong-Ying CHEN
2
;
Wen-Jun LIU
2
Author Information
1. Department of Pediatrics, The Affiliated Hospital of Southwest Medical University (Birth Defects Clinical Medical Research Center of Sichuan Province), Luzhou 646000, Sichuan Province, China,E-mail: liuchunyan9910@163.com.
2. Department of Pediatrics, The Affiliated Hospital of Southwest Medical University (Birth Defects Clinical Medical Research Center of Sichuan Province), Luzhou 646000, Sichuan Province, China.
- Publication Type:Journal Article
- Keywords:
acute lymphoblastic leukemia;
extramedullary infiltration;
fusion gene;
immunophenotype;
prognosis
- MeSH:
Child;
Humans;
Neoplasm, Residual;
Retrospective Studies;
Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics*;
Oncogene Proteins, Fusion/genetics*
- From:
Journal of Experimental Hematology
2022;30(6):1673-1678
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the detection of fusion gene in children with acute lymphoblastic leukemia (ALL) of different immunophenotypes, and analyze the relationship between fusion gene and prognosis.
METHODS:The clinical data of 86 children with ALL treated in the hospital from May 2015 to May 2020 were retrospectively analyzed, the immunophenotypes and the prognosis of children were recorded, the detection of fusion gene in ALL children with different immunophenotypes was compared, the relationship between detection of fusion gene and prognosis was analyzed.
RESULTS:The results of bone marrow immunophenotype showed that there were 13 cases of T cell type and 73 cases of B cell type in 86 children with ALL. The detection rate of fusion gene SIL-TAL1 in ALL children with T cell type was significantly higher than that in ALL children with B cell type (P<0.05). The detection rates of fusion genes BCR-ABL1, E2A-PBX1 and TEL-AML1 in ALL children with B cell type were higher than those in ALL children with T cell type, but the differences were not statistically significant (P>0.05). Followed up for 8-12 months, recurrence was taken as the end point, the average follow-up time was (10.14±1.75) months, in 86 children with ALL 15 cases recurred (17.44%). The recurrence curve drawn by Kaplan-Meier method showed that the median recurrence time of 15 children with recurrent ALL was 9 months. The proportions of positive minimal residual disease and extramedullary infiltration in the poor prognosis group were higher than those in the good prognosis group, and the differences were statistically significant (P<0.05). The detection rates of fusion genes BCR-ABL and SIL-TAL1 in the poor prognosis group were higher than those in the good prognosis group, and the differences were statistically significant (all P<0.05). Logistic regression analysis showed that positive minimal residual lesions, extramedullary infiltration, and detection of fusion genes BCR-ABL and SIL-TAL1 were risk factors for poor prognosis in children with ALL (OR>1, P<0.05). The ROC curve analysis showed that the area under the curve (AUC) of combined detection of fusion gene BCR-ABL and SIL-TAL1 for predicting the poor prognosis of ALL children was >0.707, which had a certain predictive value.
CONCLUSION:There are differences in fusion genes among ALL children with different immunophenotypes, minimal residual disease, extramedullary infiltration, and fusion gene are associated with prognosis of ALL children. Fusion gene detection can be used as new method to predict the prognosis of children with ALL.
