Effect of BECN1 Expression on Proliferation and Invasion of Human Myeloma Cell Line RPMI-8226.
10.19746/j.cnki.issn.1009-2137.2022.05.025
- Author:
Xiao-Qin LIU
1
;
Yuan-Lin XIONG
1
;
Yao MA
1
;
Hua-E SHU
2
Author Information
1. Department of Hematology, Rheumatology and Immunology, People's Hospital of Kaizhou District, Chongqing 405400, China.
2. Department of Hematology, Rheumatology and Immunology, People's Hospital of Kaizhou District, Chongqing 405400, China,E-mail: shuhuae76@126.com.
- Publication Type:Journal Article
- Keywords:
invasion;
BECN1;
myeloma cell RPMI-8226;
proliferation
- MeSH:
Beclin-1/metabolism*;
Cadherins/metabolism*;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Gene Expression Regulation, Neoplastic;
Humans;
Multiple Myeloma/genetics*;
RNA, Messenger/metabolism*;
Sincalide/metabolism*;
beta Catenin/genetics*
- From:
Journal of Experimental Hematology
2022;30(5):1469-1473
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of BECN1 expression on proliferation and invasion of human multiple myeloma (MM) cell line RPMI-8226.
METHODS:RPMI-8226 cells were cultured in vitro, the recombinant plasmid pcDNA3.1-BECN1 was constructed and transfected into RPMI-8226 cells, then the cells were divided into three groups: control group, negative transfection group and BECN1 transfection group. The expression of BECN1 mRNA in cells of each group was detected by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR); the effect of BECN1 overexpression on cell proliferation inhibition rate was detected by cell counting kit 8 (CCK-8); the effect of BECN1 overexpression on the colony formation rate was detected by plate cloning assay; the effect of BECN1 overexpression on cell invasion was detected by Transwell assay; the effects of BECN1 overexpression on the expression of cell proliferation, invasion and autophagy-related proteins were detected by Western blot.
RESULTS:The expression of BECN1 mRNA in BECN1 transfection group was significantly higher than that in control group and negative transfection group (P<0.05); the inhibition rate of cell proliferation and the expression of autophagy-related proteins Beclin1, Atg5 and invasion-related protein E-cadherin in BECN1 transfection group were significantly higher than those in control group and negative transfection group; the colony formation rate, invasion number and the expression of proliferation-related proteins CyclinD1, β-catenin and invasion-related protein N-cadherin were significantly lower than those in control group and negative transfection group (P<0.05).
CONCLUSION:Overexpression of BECN1 can inhibit the proliferation and invasion of human MM cells RPMI-8226, which may be a potential research target of MM.
