Correlation between the Promoter Methylation Status of TRIM58 and Its mRNA Expression in Acute Myeloid Leukemia.
10.19746/j.cnki.issn.1009-2137.2022.05.09
- Author:
Cheng-Kan DU
1
;
Yue JIANG
2
;
Ying LU
3
;
Xue YANG
4
Author Information
1. Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
2. The second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China.
3. Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China,E-mail：stove@shsmu.edu.cn.
4. Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
- Publication Type:Journal Article
- Keywords:
CD34;
DNA methylation;
TRIM58;
acute myeloid leukemia
- MeSH:
DNA Methylation;
Decitabine;
Humans;
Leukemia, Myeloid, Acute/metabolism*;
RNA, Messenger/metabolism*;
Tripartite Motif Proteins/metabolism*
- From:
Journal of Experimental Hematology
2022;30(5):1354-1360
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the relationship between the promoter methylation status of Tripartite-motif protein 58 (TRIM58) and its mRNA expression level in acute myeloid leukemia (AML), and to explore the expression and regulation of TRIM58 in AML.
METHODS:The bisulfite sequencing PCR (BSP) and quantitative real-time PCR (qPCR) technologies were used to detect the promoter methylation status and expression levels of TRIM58 mRNA in primary CD34+ and CD34- AML cells and the AML cell lines KG1a and K562 were determined.
RESULTS:The expression of TRIM58 mRNA in CD34+ cells was down-regulated in 10 AML patients, while that in CD34- cells was down-regulated in 12 cases. Differences in the promoter methylation level of TRIM58 were statistically significant between AML group and normal control group (P<0.05). Additionally, the expression of TRIM58 mRNA was down-regulated in cell lines KG1a and K562, and up-regulated after decitabine treatment.
CONCLUSION:The down-regulation of mRNA expression of TRIM58 in AML cells may be related to its promoter methylation status.
