Effect of Interleukin-6 Gene Deletion on Radiation-Induced Mouse Hematopoietic Injury and Relative Mechanism.
10.19746/j.cnki.issn.1009-2137.2022.04.045
- Author:
Yang-Yang DAI
1
;
Shuang XING
2
;
Hao LUAN
3
;
Jin-Kun YANG
4
;
Zi-Zhi QIAO
4
;
Xue-Wen ZHANG
4
;
Guo-Lin XIONG
4
;
Xing SHEN
4
;
Zu-Yin YU
5
Author Information
1. School of Life Science, Anhui Medical University, Hefei 230032, Anhui Province, China.
2. Department of Experimental Hematology and Biochemistry, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China,E-mail: xshuang6564@163.com.
3. School of Life Science, Anhui Medical University, Hefei 230032, Anhui Province, China,Department of Experimental Hematology and Biochemistry, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China.
4. Department of Experimental Hematology and Biochemistry, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China.
5. School of Life Science, Anhui Medical University, Hefei 230032, Anhui Province, China,Department of Experimental Hematology and Biochemistry, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, China,E-mail: yuzy79@163.com.
- Publication Type:Journal Article
- Keywords:
acute radiation sickness;
bone marrow inhibition;
hematopoietic injury;
interleukin-6;
ionizing radiation
- MeSH:
Animals;
Gene Deletion;
Granulocyte Colony-Stimulating Factor/pharmacology*;
Interleukin-6/metabolism*;
Mice;
Radiation Injuries;
Whole-Body Irradiation
- From:
Journal of Experimental Hematology
2022;30(4):1255-1261
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of interleukin-6 (IL-6) gene deletion on radiation-induced hematopoietic injury in mice and relative mechanism.
METHODS:Before and after whole body 60Co γ-ray irradiation, it was analyzed and compared that the difference of peripheral hemogram, bone marrow hematopoietic stem and progenitor cells conts in IL-6 gene knockout (IL-6-/-) and wild-type (IL-6+/+) mice and serum IL-6 and G-CSF expression levels in above- mentioned mouse were detected. Moreover, 30 days survival rate of IL-6-/- and IL-6+/+ mice after 8.0 Gy γ-ray irradiation were analyzed.
RESULTS:IL-6 levels in serum of IL-6+/+ and IL-6-/- mice were respectively (98.95±3.85) pg/ml and (18.36±5.61) pg/ml, which showed a significant statistical differences (P<0.001). There were no significant differences of peripheral blood cell counts and G-CSF level in serum between IL-6+/+ and IL-6-/- mice before irradiation (P>0.05). However, the number of leukocytes, neutrophils, lymphocytes, monocytes, platelets in peripheral blood and G-CSF level in serum of IL-6-/- mice were significantly decreased at 6 h after 8.0 Gy γ-ray irradiation compared with that of IL-6+/+ mice. On days 30 after 8.0 Gy γ-ray irradiation, the survival rate of IL-6+/+ and IL-6-/- mice was 62.5% and 12.5%, and the mean survival time of dead mice was 16.0±1.0 and 10.6±5.3 days, respectively. On days 14 after 6.5 Gy γ-ray irradiation, bone marrow nucleated cells in IL-6+/+ and IL-6-/- mice were respectively (10.0±1.2)×106 and (8.3±2.2)×106 per femur. Compared with IL-6+/+ mice, the proportion of Lin-Sca-1-c-kit+ (LK) in bone marrow of IL-6-/- mice had no significant change (P>0.05), but the proportion of Lin-Sca-1+c-kit+ (LSK) was significantly decreased (P<0.05).
CONCLUSION:IL-6 plays an obvious role in regulating hematopoietic radiation injury, and IL-6 deficiency can inhibit the radiation-induced increase of endogenous G-CSF level in serum, aggravates the damage of mouse hematopoietic stem cells（HSC） and the reduction of mature blood cells in peripheral blood caused by ionizing irradiation, resulting in the shortening of the survival time and significant decrease of the survival rate of mice exposed to lethal dose radiation.
