Correlative Analysis between Production of Platelet HLA-Ⅰ Antibody and HLA-A, B Genes in Patients with Malignant Hematological Diseases.
10.19746/j.cnki.issn.1009-2137.2022.04.036
- Author:
Xiao-Yun GAO
1
;
Li-Duo KOU
1
;
Hua TIAN
1
;
Xin-Hua WANG
2
Author Information
1. Department of Blood Transfusion, Aerospace Center Hospital, Beijing 100049, China.
2. Department of Blood Transfusion, Aerospace Center Hospital, Beijing 100049, China.E-mail: wangxinhua721@163.com.
- Publication Type:Journal Article
- Keywords:
human leukocyte antigen;
malignant hematological disease;
platelet antibody;
susceptible gene
- MeSH:
Alleles;
Antibodies;
Gene Frequency;
HLA-A Antigens/genetics*;
HLA-B Antigens/genetics*;
Hematologic Diseases/genetics*;
Humans;
Platelet Transfusion
- From:
Journal of Experimental Hematology
2022;30(4):1203-1207
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the correlation between the production of platelet HLA-Ⅰ antibody and HLA-A, B genes in patients with malignant hematological diseases, and explore the susceptible gene for producing platelet HLA-Ⅰ antibody.
METHODS:Patients with malignant hematological diseases who had received multiple platelet transfusion were selected as the research objects in the Department of Hematology of our hospital. Platelet HLA-I antibody were screened by ELISA, and the patients were divided into positive and negative groups according to the results. HLA-A and B genes were sequenced after genomic DNA was extracted, and the frequencies of them were compared between the two groups.
RESULTS:The positive rate of platelet HLA-I antibody was 22.95%. A total of 13 HLA-A alleles and 14 HLA-B alleles were obtained after the HLA-A and B genes sequencing in 100 cases. The frequencies of HLA-A*24, HLA-A*30, and HLA-B*13 were significantly different between the two groups (P<0.05). Frequencies of HLA-A*30 and HLA-B*13 in the positive group were lower than those in the negative group (RR=0.107, 0.387), but HLA-A*24 was higher (RR=1.412). After high-resolution typing of HLA-A*24, HLA-A*30, and HLA-B*13, frequencies of HLA-A*24∶02, HLA-A*30∶01, and HLA-B*13∶02 were significantly different between the two groups, the RR value was 1.412, 0.107, and 0.125, 95%CI was 0.961-2.075, 0.016-0.721, and 0.300-0.515, respectively.
CONCLUSION:HLA-A*24∶02 may be a susceptible gene for producing platelet HLA-Ⅰ antibody in patients with malignant hematological diseases, while HLA-A*30∶01 and HLA-B*13∶02 may be two protective genes.
