Gene Sequencing Analyses of 10 ABO Ambiguous Blood Group Samples.
10.19746/j.cnki.issn.1009-2137.2022.04.034
- Author:
Hui Ni HUANG
1
;
Zhu Ning MO
1
;
Xiang Cheng LIAO
1
;
Zi Ji YANG
1
;
Yue Rong WEI
1
;
Chun Li ZHU
1
;
Hai Lan LI
2
Author Information
1. Department of Blood Transfusion, Guangxi Zhuang Autonomous Region People's Hospital, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
2. Department of Blood Transfusion, Guangxi Zhuang Autonomous Region People's Hospital, Nanning 530021, Guangxi Zhuang Autonomous Region, China,Email:feiyang2808 @163.com.
- Publication Type:Journal Article
- Keywords:
ABO blood group;
ambiguous blood group;
gene sequencing
- MeSH:
ABO Blood-Group System/genetics*;
Alleles;
Blood Grouping and Crossmatching;
Exons;
Genotype;
Phenotype
- From:
Journal of Experimental Hematology
2022;30(4):1193-1197
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.
METHODS:A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.
RESULTS:The genotypes of 10 ABO ambiguous blood group samples were A102/BW11, A102/BW12, O02/O02, A102/B303, A102/B101, BW11/O02, B101/O04, BW11/O01, BW11/O01, A101/O02, respectively. The genotype results of 6 cases was consistent with the serological phenotype, and the serological phenotype of 4 cases were different from the geno sequencing.
CONCLUSION:ABO blood groups genotyping technology combined with serological typing can be used for accurate typing of ambiguous blood group, and better ensure the safety of blood transfusion.
