Release of Exosomes Derived from Leukocyte-Depleted Red Cell Suspension and Its Regulation on Hematological Tumor Cells.
10.19746/j.cnki.issn.1009-2137.2022.04.033
- Author:
Hao-Bo HUANG
1
;
Li-Ping FAN
1
;
Qiu-Yan LIN
1
;
Hui-Wen HUANG
1
;
Dan-Hui FU
2
Author Information
1. Department of Blood Transfusion, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China.
2. Department of Blood Transfusion, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China,Department of Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China,E-mail: fudanhui@189.cn.
- Publication Type:Journal Article
- Keywords:
exosome;
leukocyte-depleted red cell suspension;
proliferation;
tumor cell
- MeSH:
Cell Proliferation;
Erythrocytes;
Exosomes/metabolism*;
Hematologic Neoplasms/metabolism*;
Humans;
Leukocytes
- From:
Journal of Experimental Hematology
2022;30(4):1188-1192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the release of exosome (Exo) from leukocyte-depleted red cell suspension (LDRCS) at different storage time and its regulation on proliferation of hematological tumor cells and possible mechanism.
METHODS:The Exo (RBC-Exo) in LDRCS at different storage time was obtained by ultracentrifugation, and the morphology and immunological marker of RBC-Exo were detected by transmission electron microscopy and Western blot, respectively. The particle size distribution of RBC-Exo in LDRCS at different storage time was detected by Dynamic Light Scattering. CCK-8 assay was used to explore the effect of RBC-Exo on hematological tumor cell proliferation. Western blot was used to detect the expression of proliferation-related proteins in hematological tumor cells after co-culture with RBC-Exo.
RESULTS:RBC-Exo was isolated, which was characterized by cup-like shape, particle size distribution ranged from 20 to 200 nm, CD63/TSG101 enriched, Calnexin negative, CD235a positive and CD41 negative. The particle size distribution of RBC-Exo from LDRCS between middle was not significantly different and late stored stage. But the particle size distribution of RBC-Exo at middle-late stored stage(>14 d) was larger than that at early stored stage (≤14 days). Compared with the control group, RBC-Exo could significantly promote the proliferation of HBL1, U2932 and Jurkat cells. Compared with the control group, the cycle-related protein P21 was significantly down-regulated in HBL1, U2932 and Jurkat cells after co-culture with RBC-Exo for 3 days, while the anti-apoptotic protein BCL-2 was not changed significantly.
CONCLUSION:The morphology of RBC-Exo from LDRCS at middle-late stored stage was different from that at early stored stage. RBC-Exo could promote the proliferation of hematological tumor cells, possibly by regulating the expression of cycle-associated protein P21.
