Apoptosis of Megakaryocytic Leukemia Cell Line Meg-01 Induced by TSP-1 Via CD36/Caspase-3.
10.19746/j.cnki.issn.1009-2137.2022.04.004
- Author:
Hui-Min KONG
1
;
Wei-Qing SU
2
;
Yi LUO
2
;
Hui GE
3
;
Liang LI
4
;
Mo YANG
5
,
6
;
Qian-Li JIANG
7
Author Information
1. Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
2. Department of Oncology Hematology, Lianjiang People's Hospital, Zhanjiang 524400, Guangdong Province, China.
3. Department of Pediatrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
4. Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, Guangdong Province, China.
5. Department of Oncology Hematology, Lianjiang People's Hospital, Zhanjiang 524400, Guangdong Province, China
6. Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, Guangdong Province, China.
7. Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China . E-mail: 1420272692@qq.com.
- Publication Type:Journal Article
- Keywords:
CD36;
Meg-01;
apoptosis;
megakaryocytic leukemia;
thrombospondin-1
- MeSH:
Animals;
Apoptosis;
CD36 Antigens/metabolism*;
Caspase 3/metabolism*;
Cell Line;
Humans;
Leukemia, Megakaryoblastic, Acute;
Mice;
Thrombospondin 1/pharmacology*
- From:
Journal of Experimental Hematology
2022;30(4):998-1004
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of thrombospondin-1 (TSP-1) on apoptosis of human megakaryocytic leukemia cell line Meg-01 and its possible mechanism.
METHODS:The expression of CD36 antigen in Meg-01 cells was detected by flow cytometry and immunocytochemistry. Meg-01 cells were cultured for 48 hours with TSP-1 and CD36 antibody FA6-152 at different concentrations. The early apoptosis and activity of caspase-3 were detected by flow cytometry. The effect of TSP-1 on the growth and differentiation of megakaryocytes was investigated by cell counting and CFU-MK culture.
RESULTS:The flow cytometry and immunocytochemistry showed that CD36 antigen was expressed on the surface of Meg-01 cells. TSP-1 (5 μg/ml) inhibited the growth of Meg-01 cells, but had unobvious effect on M-07e cells. After addition of CD36 antibody FA6-152 (5, 10, and 25 μg/ml), the inhibition effect of TSP-1 was significantly reduced. TSP-1 (2.5, 5, and 7.5 μg/ml) increased the positive expression of Annexin V (P<0.01) and caspase-3 activity (P<0.01), which indicated that TSP-1 had a significant effect on inducing apoptosis. After addition of CD36 antibody FA6-152 (25 μg/ml), the apoptosis induced by TSP-1 in Meg-01 cells was significantly reduced. TSP-1 (5, 10, and 25 μg/ml) could significantly inhibit the formation of CFU-MK in mouse bone marrow cells, while β-TG could not. CD36 antibody FA6-152 (25 μg/ml) could significantly reduce the inhibition of TSP-1 on CFU-MK.
CONCLUSION:TSP-1 may induce apoptosis of megakaryocytic leukemia cell line Meg-01 cells via CD36/caspase-3, which provides a potential new drug development and treatment target for clinical treatment of megakaryocytic leukemia.
