Update on techniques for cryopreservation of human spermatozoa.

Chuan Huang; Yu-lin Tang; Jian-ling HU; Wen-jun Zhou; Zeng-hui Huang; Xue-feng Luo; Zheng LI; Wen-bing Zhu

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Update on techniques for cryopreservation of human spermatozoa.

Author: Chuan HUANG ¹ ; Yu-Lin TANG ¹ ; Jian-Ling HU ² ; Wen-Jun ZHOU ¹ ; Zeng-Hui HUANG ¹ ; Xue-Feng LUO ¹ ; Zheng LI ² ; Wen-Bing ZHU ¹
Author Information

1. Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Changsha 410000, China.
2. Department of Andrology, Center for Men's Health, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai 200025, China.
Publication Type:Review
Keywords: cryodamage; cryoprotectant; dry preservation; freezing and thawing; sperm cryopreservation
MeSH: Humans; Male; Semen Preservation/methods*; Sperm Motility; Semen; Cryopreservation/methods*; Spermatozoa; Cryoprotective Agents/pharmacology*
From: Asian Journal of Andrology 2022;24(6):563-569
CountryChina
Language:English
Abstract: In the 1960s, sperm cryopreservation was developed as a method to preserve fertility. Currently, techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction. However, although sperm cryobiology has made notable achievements, the optimal method for the recovery of viable spermatozoa after cryopreservation remains elusive. Postthawing sperm quality can be affected by cryoprotectants, ice formation, storage conditions, and osmotic stress during the freezing process. This review discusses recent advances in different cryopreservation techniques, cryoprotectants, and freezing and thawing methods during cryopreservation and new indications for the use of cryopreserved spermatozoa.