A multiplex PCR-based sensitive and specific method for detecting Y chromosome material in patients with Turner syndrome.
10.3760/cma.j.cn511374-20211209-00978
- Author:
Qiang ZHAO
1
;
Shuxiong CHEN
;
Hailin SUN
;
Wanling YANG
;
Bo BAN
Author Information
1. Cheeloo College of Medicine, Shandong University, Ji'nan, Shandong 250100, China. banbo2011@163.com.
- Publication Type:Journal Article
- MeSH:
Humans;
Male;
Turner Syndrome/genetics*;
Multiplex Polymerase Chain Reaction;
Y Chromosome;
Karyotyping;
DNA Primers;
DNA;
Chromosomes, Human, Y/genetics*;
Transducin/genetics*;
Minor Histocompatibility Antigens;
DEAD-box RNA Helicases/genetics*
- From:
Chinese Journal of Medical Genetics
2022;39(11):1216-1223
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a multiplex PCR method for a rapid detection of Y chromosome-specific sequences in patients with Turner syndrome.
METHODS:Nine genes were selected from various regions of the Y chromosome for designing the primers, which included SRY, TBL1Y, TSPY on the short arm of the Y chromosome, DDX3Y, HSFY1, RPS4Y2 and CDY1 on the long arm of Y chromosome and SHOX in the short arm and SPRY3 in the long arm of the pseudoautosomal region (PAR) of X and Y chromosomes. A multiplex PCR method for the nine genes in Y chromosome was established and optimized. The sensitivity was tested by using different amounts of genomic DNA. A total of 36 patients with Turner syndrome and a patient with male dwarfism with karyotype of 46, X, +mar were examined by the multiplex PCR method for the existence of materials from the Y chromosome.
RESULTS:The optimization results of the multiplex PCR reaction system (50 μL) showed that when the final concentration of upstream and downstream of each pair of primers was 0.1 μM, the multiplex PCR reaction of the 9 pairs of primers clearly amplified the target with the expected band size, and there was no non-specific amplification. The bands were clearly visible when the amount of genomic DNA in the multiple PCR reaction system was as low as 1 ng. By using the method, we have examined the 36 patients with Turner syndrome. One patient with Turner syndrome with karyotype of 45,X[40]/47XYY[21] amplified specific seven genes on Y chromosome, 35 patients with Turner syndrome amplified only two target genes SHOX and SPRY3, but not the other seven specific genes on the Y chromosome, which was in keeping with the clinical manifestations of such patients.
CONCLUSION:This study established a multiplex PCR reaction system with nine genes, which can quickly and accurately screen Y chromosome materials in patients with Turner syndrome. It has the advantages of low cost, simple operation, high specificity and rapid turn-around time, and can be used to detect Turner syndrome patients with Y chromosome material in time. The method has provided a diagnostic basis for preventive gonad resection to prevent malignant gonadal tumors.
