Clinical significance of serum miRNA-146, OX-LDL and ROS expression in patients with primary ovarian insufficiency.
10.3760/cma.j.cn511374-20211018-00823
- Author:
Yanhui LIU
1
;
Jia'nan LIU
;
Fu XIONG
;
Yan SUN
;
Jianhua LUO
;
Peiqing HE
;
Fengping HE
Author Information
1. Dongguan Maternal and Child Health Care Hospital, Dongguan, Guangdong 523110, China. watering@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Humans;
Female;
Mice;
Animals;
Toll-Like Receptor 4/metabolism*;
NF-kappa B/metabolism*;
MicroRNAs/metabolism*;
Reactive Oxygen Species/pharmacology*;
Primary Ovarian Insufficiency/genetics*;
Apoptosis/genetics*
- From:
Chinese Journal of Medical Genetics
2022;39(11):1211-1215
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the clinical significance of miRNA-146, OX-LDL and ROS in patients with primary ovarian insufficiency (POI).
METHODS:100 patients with POI were prospectively collected and 100 women with normal ovarian function were randomly selected as control group. Serum miRNA-146 expression level was detected by qRT-PCR and serum OX-LDL and ROS expression levels were detected by ELISA. Ovarian granulosa cells of mouse were transfected with miRNA-146 mimics or inhibitors, and then treated with OX-LDL. Cell viability, colony forming ability, apoptosis rate and toll like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) of pathway proteins were evaluated respectively.
RESULTS:Compared with control group, the expression level of miRNA-146 in POI group was significantly lower, the expression level of OX-LDL and ROS were significantly higher, and the ovarian volume and peak systolic blood flow velocity of ovarian artery were significantly decreased in POI group. Upregulation of miRNA-146 expression had a protective effect on OX-LDL injured ovarian granulosa cells, as evidenced by increased ovarian granulosa cell viability and colony number, reduced apoptosis, and downregulation of TLR4/NF-κB expression.
CONCLUSION:miRNA-146 can target downstream TLR4/NF-κB signaling pathway affects oxidative stress and inflammatory response of POI induced by OX-LDL and ROS, and is expected to become a biomarker for early prediction of POI and a new target for treatment.
