Effects of different drying methods on ginsenosides based on UHPLC-Q-Exactive Orbitrap MS technique.
10.19540/j.cnki.cjcmm.20220106.302
- Author:
Hong WANG
1
;
Yi-Fang CUI
1
;
Hui-Zhen JIANG
2
;
Shao-Ping WANG
3
;
Xiao-Guang SONG
2
;
Li-Juan HAN
4
;
Long DAI
3
;
Ning LI
4
;
Jia-Yu ZHANG
3
Author Information
1. School of Pharmacy, Binzhou Medical University Yantai 264003, China College of Pharmacy, Shandong University of Traditional Chinese Medicine Ji'nan 250300, China.
2. Yantai Testing Center for Food and Drug Yantai 264003, China.
3. School of Pharmacy, Binzhou Medical University Yantai 264003, China.
4. Kangmeihuada Gene Technology Co., Ltd. Shenzhen 518000, China.
- Publication Type:Journal Article
- Keywords:
Panax ginseng;
UHPLC-Q-Exactive Orbitrap MS;
drying methods;
ginsenosides
- MeSH:
Ginsenosides/analysis*;
Chromatography, High Pressure Liquid/methods*;
Panax;
Mass Spectrometry/methods*;
Saponins
- From:
China Journal of Chinese Materia Medica
2022;47(19):5246-5255
- CountryChina
- Language:Chinese
-
Abstract:
The present study quickly identified the ginsenosides in fresh Panax ginseng and specified the effects of different drying methods(50 ℃-drying, 80 ℃-drying, and-70 ℃ freeze-drying) on ginsenosides.Three P.ginseng products by different drying methods were prepared, and the UHPLC-Q-Exactive Orbitrap high-resolution liquid mass spectrometry(MS) technique was applied to perform gradient elution using water-acetonitrile as the mobile phase, and the data collected in the negative ion mode were analyzed using X Calibur 2.2.The results showed that 57 saponins were identified from fresh P.ginseng.As revealed by the comparison with the fresh P.ginseng, in terms of the loss of ginsenosides, the dried products were ranked as the dried product at 50 ℃, freeze-dried products at-70 ℃, and the dried product at 80 ℃ in the ascending order.This study elucidated the effects of different drying methods on the types and relative content of ginsenosides, which can provide references for the processing of P.ginseng in the producing areas.
