Detection of chondroitin sulfate in Cervi Cornu Pantotrichum and Cervi Cornu of different specifications and its application in quality evaluation.
10.19540/j.cnki.cjcmm.20220616.101
- Author:
Yu-Shun LU
1
;
Yan-Ting ZHANG
1
;
Zhi-Man LI
1
;
Jian-Bo CHEN
1
;
Xiao-Hui HUO
1
;
Di QU
1
;
Yin-Shi SUN
1
Author Information
1. Institute of Special Animal and Plant Sciences, China Academy of Agricultural Sciences Changchun 130112, China.
- Publication Type:Journal Article
- Keywords:
Cervi Cornu;
Cervi Cornu Pantotrichum;
chondroitin sulfate;
high-performance liquid chromatography;
quality evaluation
- MeSH:
Animals;
Cornus;
Chondroitin Sulfates;
Deer;
Powders;
Antlers;
Gastropoda
- From:
China Journal of Chinese Materia Medica
2022;47(19):5203-5208
- CountryChina
- Language:Chinese
-
Abstract:
The present study comprehensively compared the content of chondroitin sulfate in Cervi Cornu Pantotrichum(CCP) and Cervi Cornu(CC) of different specifications and explored the feasibility of chondroitin sulfate as an indicator to distinguish between CCP and CC. Twenty-two batches of CCP of different specifications(two-branched velvet antler and three-branched velvet antler) from 15 habitats, CC from 6 habitats, and 60 batches of CCP slices prepared from different parts(wax slices, powder slices, gauze slices, and bone slices) were collected. High-performance liquid chromatography(HPLC) was used to determine chondroitin sulfate content in CCP and CC of different specifications. Cluster analysis was used to classify CCP slices of different specifications. The results showed that CCP contained abundant chondroitin sulfate. The average content of chondroitin sulfate was 2.35 mg·g~(-1) in two-branched velvet antler and 1.79 mg·g~(-1) in three-branched velvet antler, significantly higher than 0.11 mg·g~(-1) in CC. Chondroitin sulfate content in wax slices, powder slices, gauze slices, and bone slices were 7.81, 8.39, 1.33, and 0.54 mg·g~(-1), respectively. Cluster analysis showed that gauze slices and bone slices could be clustered into one category and distinguished from wax slices and powder slices. CCP slices prepared from different parts could be separated well through chondroitin sulfate content. Based on the five principles of Q-marker selection, chondroitin sulfate can be used as a potential Q-marker for the identification of CCP and CC, as well as a potential quality indicator for CCP slices of different specifications(wax slices, powder slices, gauze slices, and bone slices). This research provides data support for CCP quality evaluation.
