Terpenoids from fruits of Amomum villosum and their hypoglycemic activity.
10.19540/j.cnki.cjcmm.20220411.203
- Author:
Min DING
1
;
Sheng-Li WU
2
;
Xiao-Feng HE
3
;
Xue-Mei ZHANG
3
;
Chang-An GENG
3
Author Information
1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany,Chinese Academy of Sciences Kunming 650201, China University of Chinese Academy of Sciences Beijing 100049, China.
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany,Chinese Academy of Sciences Kunming 650201, China School of Life Sciences, Yunnan University Kunming 650500, China.
3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany,Chinese Academy of Sciences Kunming 650201, China.
- Publication Type:Journal Article
- Keywords:
GLP-1 stimulation;
fruits of Amomum villosum;
hypoglycemic effect;
inhibitory activity against GPa;
kravanhin A 3-O-β-D-glucopyranoside
- MeSH:
Fruit/chemistry*;
Terpenes/analysis*;
Amomum;
Hypoglycemic Agents/analysis*;
Chromatography, High Pressure Liquid
- From:
China Journal of Chinese Materia Medica
2022;47(21):5849-5854
- CountryChina
- Language:Chinese
-
Abstract:
Eight terpenoids were isolated from the fruits of Amomum villosum by silica gel, Sephadex LH-20, Rp-C_(18), MCI GEL CHP20 P column chromatography, preparative TLC, and HPLC. Their structures were identified by HR-ESI-MS, ~1H and ~(13)C-NMR, IR, UV, [α]_D, and ECD spectroscopic data as kravanhin A 3-O-β-D-glucopyranoside(1), kravanhin B(2), 6-eudesmene-1β,4β-diol(3), oplodiol(4), vicodiol(5),(1R,2S,4R,7S)-vicodiol 9-O-β-D-glucopyranoside(6),(1R,2S,4S,5R)-angelicoidenol 2-O-β-D-glucopyranoside(7), and(1S,2S,4R,6S)-bornane-2,6-diol 2-O-β-D-glucopyranoside(8). Compound 1 was a new compound, and compounds 2-5 were isolated from A. villosum for the first time. Their hypoglycemic activity was tested based on STC-1 cell model and two enzymatic models(GPa and PTP1 B). The results showed that compounds 1, 7, and 8 could stimulate GLP-1 with the secretion rates of 692.8%, 398.6%, and 483.3% at 25.0 μmol·L~(-1), and compound 6 showed inhibitory activity against GPa with an IC_(50) value of 78.6 μmol·L~(-1).
