Effective delivery of quality markers in alcohol precipitation of Pudilan Xiaoyan Oral Liquid.
10.19540/j.cnki.cjcmm.20220520.302
- Author:
Ran HUANG
1
;
Meng-Hua JIANG
2
;
E SUN
2
;
Chao LI
3
;
Fa-Gen ZHU
3
;
Jian-Guo SHAO
3
;
Liang FENG
4
;
Xiao-Bin JIA
4
Author Information
1. the Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210029, China Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Province Academy of Traditional Chinese Medicine Nanjing 210028,China.
2. Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Province Academy of Traditional Chinese Medicine Nanjing 210028,China.
3. Jiangsu Key Laboratory of Chinese Medicine and Characteristic Preparations for Paediatrics, Jumpcan Pharmaceutical Co., Ltd. Taixing 225400, China.
4. School of Traditional Chinese Pharmacy, China Pharmaceutical University Nanjing 211198, China.
- Publication Type:Journal Article
- Keywords:
Chinese medicine oral liquid preparations;
Pudilan Xiaoyan Oral Liquid;
alcohol precipitation;
effective delivery;
quality markers
- MeSH:
Drugs, Chinese Herbal;
Ethanol;
Adenosine;
Chemical Precipitation
- From:
China Journal of Chinese Materia Medica
2022;47(21):5757-5764
- CountryChina
- Language:Chinese
-
Abstract:
Taking Pudilan Xiaoyan Oral Liquid as a demonstration, the effective delivery of quality markers in alcohol precipitation of Chinese medicine oral liquid preparations was studied. With the transfer rates of adenosine, corynoline, cichoric acid, baicalin, and wogonin as evaluation indexes, the effect of the density of concentrate before alcoholic precipitation, volume fraction of ethanol, stirring speed, temperature of concentrated solution, stirring time, alcohol concentration, alcohol precipitation time, alcoholic precipitation temperature, alcohol addition rate, and the pH of concentrate on the alcohol precipitation process was investigated by Plackett-Burman trial design, thus obtaining the key factors that influenced the alcohol precipitation process. The key factors were further optimized by Box-Behnken design to determine the optimal alcohol precipitation conditions. When the density of concentrate before alcoholic precipitation was 1.12 g·mL~(-1), the pH of concentrate was 6.86, and the alcohol concentration was 50.00%, the transfer rates of baicalin and wogonin were 91.86% and 87.78%, respectively. When the density of concentrate before alcoholic precipitation was 1.13 g·mL~(-1), the concentration of alcohol was 74.50%, and the alcoholic precipitation temperature was 17.0 ℃, the transfer rates of adenosine, corynoline, and cichoric acid were 85.95%, 71.62% and 83.19%, respectively. The method of optimizing alcohol precipitation techniques and determining the parameters of Pudilan Xiaoyan Oral Liquid by response surface methodology is reasonable and feasible, which provides guidance and experience for the effective delivery of quality markers in Chinese medicine oral liquid preparations.
