Digestive properties of flavonoid components in Xiaoer Chiqiao Qingre Granules in simulated infant gastrointestinal environment.
10.19540/j.cnki.cjcmm.20220614.301
- Author:
Bin-Bin ZHOU
1
;
Yan-Jun YANG
1
;
Mao-Mao ZHU
1
;
Bing YANG
1
;
Wei-Kun MA
1
;
Xiang-Jun ZHU
2
;
Jun LIU
3
;
Jing ZHAO
3
;
Liang FENG
1
;
Xiao-Bin JIA
1
Author Information
1. State Key Laboratory of Natural Medicine Active Components and Pharmacodynamics, School of Traditional Chinese Pharmacy,China Pharmaceutical University Nanjing 211198, China.
2. Jiangsu Health Development Research Center, National Health and Family Planning Commission Contraceptives Adverse Reaction Surveillance Center Nanjing 210036, China.
3. Jiangsu Key Laboratory of Chinese Medicine and Characteristic Preparations for Paediatrics, Jumpcan Pharmaceutical Co., Ltd. Taixing 225400, China.
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF-MS;
Xiaoer Chiqiao Qingre Granules;
digestive characteristics;
flavonoid components;
infants
- MeSH:
Infant;
Humans;
Child;
Flavonoids/metabolism*;
Gastrointestinal Tract;
Intestines;
Drugs, Chinese Herbal/metabolism*;
Chromatography, High Pressure Liquid
- From:
China Journal of Chinese Materia Medica
2022;47(21):5735-5745
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to elucidate the digestive characteristics of flavonoid components in Xiaoer Chiqiao Qingre Granules(XECQ) in the gastrointestinal environment of infants. An in vitro model was established to simulate the gastric and intestinal environment of infants. UPLC was used to analyze the content change of flavonoid components in XECQ, and their overall content was integrated through the mass fraction weight coefficient method. UPLC-Q-TOF-MS was employed to determine the digestive products of flavonoid components in gastrointestinal fluids and their metabolic pathways. The results showed that in the process of digestion, 11 digestion products were generated by oxidation, reduction, deglycosylation, methylation and other phase Ⅰ metabolism. From flavonoid content and component changes, it was found that the flavonoid components in XECQ were relatively stable in the gastric fluid, while their content in the intestinal fluid was first increased and then maintained stable. This was mainly because flavonoid components were released from proteins, polysaccharides and other macromolecular substances during gastrointestinal digestion. In addition, phase Ⅰ metabolism occurred, but with relatively low metabolic rate, resulting in their stable content. This study preliminarily explored the digestive characteristics of flavonoid components in XECQ in the infant gastrointestinal environment, which laid a foundation for further studying the absorption, transport and metabolism of pharmacodynamics components in XECQ, and facilitated the study of the biopharmaceutical pro-perties of pediatric Chinese medicine.
