Effects of pH value on stachydrine biosynthesis of hydroponic Leonurus japonicus and its physiological mechanism.
10.19540/j.cnki.cjcmm.20220712.101
- Author:
Yan ZHANG
1
;
Xin-Ping CUI
1
;
Wen-Quan WANG
2
;
Jun-Ling HOU
3
;
Bin-Bin YAN
1
Author Information
1. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700,China.
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.
3. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
- Publication Type:Journal Article
- Keywords:
Leonurus japonicus;
Schiff base formation reaction;
biosynthesis;
intermediate products;
pH value;
reductive ammoniation reaction;
stachydrine
- MeSH:
Leonurus/chemistry*;
Hydroponics;
Alkaloids;
Nitrogen;
Hydrogen-Ion Concentration
- From:
China Journal of Chinese Materia Medica
2022;47(20):5502-5507
- CountryChina
- Language:Chinese
-
Abstract:
The present study explored the physiological mechanism of the effects of different pH treatments on the growth, physiological characteristics, and stachydrine biosynthesis of Leonurus japonicus to provide references for the cultivation and quality control of L. japonicus. Under hydroponic conditions, different pH treatments(pH 5,6,7,8) were set up. The growth, physiology, and the content of stachydrine and total alkaloids of L. japonicus, as well as the content of key intermediate products in stachydrine biosynthesis pathway(i.e., pyruvic acid, α-ketoglutaric acid, glutamic acid, and ornithine) were monitored to explore the physiological mechanism of the effects of pH on the growth and active components of L. japonicus. The results showed that L. japonicus. could grow normally in the pH 5-8 solution. The pH treatment of neutral acidity was more conducive to the accumulation of photosynthetic pigments and the increase in soluble protein in leaves of L. japonicus. to promote its growth and yield. However, since stachydrine is a nitrogen-containing pyrrolidine alkaloid, its synthesis involves the two key rate-limiting steps of nitrogen addition: reductive ammoniation reaction and Schiff base formation reaction. High pH treatments promote the synthesis and accumulation of substrates and products of the above two reactions, indicating that the alkaline environment can promote the nitrogen addition reaction, thereby promoting the biosynthesis and accumulation of stachydrine.
