Intervention effect of Jingfang Mixture on urticaria mice based on NF-κB/NLRP3/IL-1β signaling pathway.
10.19540/j.cnki.cjcmm.20220530.401
- Author:
Shi-Rong LI
1
;
Xi-Shuang WANG
1
;
Guo-Liang CHENG
2
;
Cheng-Hong SUN
1
;
Yan-Fang LI
3
;
Ru-Jing YUE
1
;
Zhen ZENG
1
;
Jing-Chun YAO
1
Author Information
1. Center for New Drug Pharmacology, Lunan Pharmaceutical Group Co., Ltd. Linyi 273400, China State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine Linyi 276006, China.
2. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine Beijing 100029, China.
3. State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine Linyi 276006, China.
- Publication Type:Journal Article
- Keywords:
IL-1β;
Jingfang Mixture;
NF-κB;
NLRP3;
inflammation;
urticaria mice
- MeSH:
Animals;
Male;
Mice;
NF-kappa B/metabolism*;
Interleukin-1beta/metabolism*;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
Tumor Necrosis Factor-alpha/metabolism*;
Ovalbumin;
Aluminum Hydroxide/pharmacology*;
Signal Transduction;
Caspase 1/metabolism*;
Urticaria;
Pruritus
- From:
China Journal of Chinese Materia Medica
2022;47(20):5467-5472
- CountryChina
- Language:Chinese
-
Abstract:
This study explored the curative effect of Jingfang Mixture on urticaria mice induced by aluminum hydroxide/ovalbumin, and discussed its mechanism. Sixty male Kunming mice were randomly divided into a normal group, a model group, three Jingfang Mixture(low-dose, medium-dose, and high-dose) groups, and a positive drug(cetirizine hydrochloride) group. The urticarial model in mice was induced by the intraperitoneal injection of the mixed solution of ovalbumin and aluminum hydroxide. The degrees of pruritus were observed after the second immunization. Pathological changes were detected by hematoxylin and eosin(HE) staining. Levels of interleukin 1β(IL-1β) and tumor necrosis factor α(TNF-α) in the serum were detected by enzyme linked immunosorbent assay(ELISA). Expressions of NOD-like receptor protein 3(NLRP3) and IL-1β were detected by immunohistochemistry(IHC). Expressions of nuclear factor kappa-B(NF-κB p65), NLRP3, apoptosis-associated speck-like protein containing a CARD(ASC), cysteinyl aspartate-specific proteases 1(caspase-1), and IL-1β proteins were detected by Western blot. The results showed that, except for the normal group, the mice in all groups had different degrees of pruritus. Compared with the model group, the Jingfang Mixture groups and the positive drug group prolonged the scratching latency of mice(P<0.05), and significantly reduced the number of scratching(P<0.05). In addition, the Jingfang Mixture groups and the positive drug group improved the pathological morphology of skin tissue. The expression levels of IL-1β and TNF-α in serum were significantly reduced(P<0.05), and the number of NLRP3 and IL-1β positive cells was decreased(P<0.01). The expressions of p-NF-κB p65, NLRP3, ASC, cleaved caspase-1, and IL-1β protein were significantly down-regulated(P<0.05). The results of the above study indicate that Jingfang Mixture inhibit the inflammatory response in urticaria mice, and the mechanism may be related to the inhibition of activating NF-κB/NLRP3/IL-1β signaling pathway.
