Mechanism of Shenling Baizhu Powder in alleviation of ulcerative colitis in mice based on high-throughput transcriptome sequencing.
10.19540/j.cnki.cjcmm.20220801.401
- Author:
Juan SUN
1
;
Xiao-Juan JIANG
2
;
Ya-Dong WANG
2
;
Ke-Long MA
2
;
Zi-Hui LI
1
;
Tian-Cheng WANG
2
;
Tian-Ming WANG
2
;
Jing SHAO
2
;
Chang-Zhong WANG
3
Author Information
1. School of Traditional Chinese Medicine, Anhui University of Chinese Medicine Hefei 230012, China.
2. School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine Hefei 230012, China.
3. School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine Hefei 230012, China Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine Hefei 230012, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui University of Chinese Medicine Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
Shenling Baizhu Powder;
cytokines;
immune regulation;
transcriptome sequencing;
ulcerative colitis
- MeSH:
Animals;
Mice;
Colitis, Ulcerative/genetics*;
Colon;
Dextran Sulfate/adverse effects*;
Disease Models, Animal;
Interleukin-10/genetics*;
Interleukin-4/genetics*;
Interleukin-6/genetics*;
Mice, Inbred C57BL;
Powders;
Transcriptome;
Tumor Necrosis Factor-alpha/metabolism*;
Drugs, Chinese Herbal/therapeutic use*
- From:
China Journal of Chinese Materia Medica
2022;47(22):6155-6163
- CountryChina
- Language:Chinese
-
Abstract:
High-throughput transcriptome sequencing was used to study the mechanism of Shenling Baizhu Powder(SLBZP) in the alleviation of the dextran sulfate sodium(DSS)-induced ulcerative colitis(UC) in mice. The mouse model of DDS-induced UC was treated with SLBZP by gavage. The changes in general state, disease activity index(DAI), and colon length were observed. The hematoxylin-eosin(HE) staining was used to observe the pathological changes in the colon tissues of mice. Enzyme-linked immunosorbent assay(ELISA) was used to determine the expression levels of tumor necrosis factor-α(TNF-α), interleukin(IL)-1β, IL-6, IL-4, and IL-10 in the serum and tissues of mice. The differentially expressed genes in the control group, the model group, and the SLBZP group were analyzed by high-throughput transcriptome sequencing, and the Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses were conducted on the differentially expressed genes. The results showed that after intragastric administration of SLBZP, the symptoms of diarrhea and bloody stool were improved, and the disease active index(DAI) score was reduced. SLBZP effectively reduced the inflammatory cell infiltration and goblet cell loss in the colonic mucosal tissue, reduced the levels of TNF-α, IL-1β, IL-6 in the serum and colon tissue, and increased the levels of IL-4 and IL-10 in the serum and colon tissue. There were 25 differential genes in SLBZP vs the model group, which were significantly enriched in immune response, immune system process, immunoglobulin production, and other biological processes. KEGG pathway analysis showed that the differential genes were enriched in signaling pathways such as neomycin, kanamycin, and gentamicin biosynthesis, cytokine-cytokine receptor interaction, primary immunodeficiency, and IgA synthesis of the intestinal immune network. This study shows that SLBZP may alleviate UC through immune regulation.
