Synchronous evaluation of continuous dynamic dissolution and absorption of Compound Danshen Dropping Pills based on cellular bioelectrical effect.
10.19540/j.cnki.cjcmm.20211217.301
- Author:
Li-Xia MA
1
;
Yi-Meng ZHAO
1
;
Yue ZHOU
1
;
Jia ZHANG
1
;
Xi YUAN
1
;
Ruo-Yan ZHANG
1
;
Jie DONG
1
;
Jun CHEN
1
;
Guo-Jun YAN
1
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
Compound Danshen Dripping Pills;
absorption kinetic model;
dissolution in vitro;
dissolution kinetic model;
pharmaceutical effect of animal;
real-time cell-based assay
- MeSH:
Rats;
Mice;
Animals;
Salvia miltiorrhiza;
Solubility;
Drugs, Chinese Herbal;
Myocytes, Cardiac
- From:
China Journal of Chinese Materia Medica
2022;47(22):6090-6096
- CountryChina
- Language:Chinese
-
Abstract:
The real-time cell-based assay(RTCA) was used to establish the bioelectrical sensing model of Compound Danshen Dripping Pills with rat cardiomyocytes(H9 c2). The time/dose-dependent cell response profiles(TCRPs) of in vitro dissolution and absorption of the pills were determined to establish the continuous dynamic dissolution and absorption kinetic models. Thereby, the cell index(CI)-based dissolution and absorption kinetic curves and kinetic models of Compound Danshen Dripping Pills were obtained. The optimal dissolution kinetic model was Weibull model. The similarity factors f_2 of dissolution curves were greater than 50 and the correlation coefficients of absorption curves were larger than 0.95. With the experiment about the efficacy on mice, percentages of the bleeding time of mice administrated with Compound Danshen Dripping Pills were calculated, and there was a correlation among dissolution, absorption, and efficacy curves(r > 0.9). RTCA is applicable to the study of the dissolution and absorption kinetics of solid compound Chinese medicine preparations. Thus, it is an innovative and feasible method to evaluate the quality and batch consistency of compound Chinese medicine preparations.
