Comparison of transcriptome of Atractylodes lancea rhizome and exploration of genes for sesquiterpenoid biosynthesis.
10.19540/j.cnki.cjcmm.20220615.101
- Author:
Ye CAO
1
;
Wen-Jin ZHANG
2
;
Li-Kun CHANG
3
;
Chuan-Zhi KANG
2
;
Yue-Feng WANG
2
;
Dong-Mei XIE
4
;
Sheng WANG
2
;
Lan-Ping GUO
1
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
4. School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
Atractylodes lancea rhizome;
sesquiterpenoid biosynthesis;
transcriptome analysis
- MeSH:
Atractylodes/genetics*;
Mevalonic Acid/metabolism*;
Rhizome/genetics*;
Sesquiterpenes/metabolism*;
Transcriptome;
Triterpenes/metabolism*
- From:
China Journal of Chinese Materia Medica
2022;47(18):4895-4907
- CountryChina
- Language:Chinese
-
Abstract:
This study compared the transcriptome of Atractylodes lancea rhizome at different development stages and explored genes encoding the key enzymes of the sesquiterpenoid biosynthesis pathway. Specifically, Illumina NovaSeq 6000 was employed for sequencing the cDNA libraries of A. lancea rhizome samples at the growth stage(SZ), flowering stage(KH), and harvesting stage(CS), respectively. Finally, a total of 388 201 748 clean reads were obtained, and 16 925, 8 616, and 13 702 differentially expressed genes(DEGs) were identified between SZ and KH, KH and CS, and SZ and CS, separately. Among them, 53 genes were involved in the sesquiterpenoid biosynthesis pathways: 9 encoding 6 enzymes of the mevalonic acid(MVA) pathway, 15 encoding 7 enzymes of the 2-C-methyl-D-erythritol-4-phosphate(MEP) pathway, and 29 of sesquiterpenoid and triterpenoid biosynthesis pathway. Weighted gene co-expression network analysis(WGCNA) yielded 12 genes related to sesquiterpenoid biosynthesis for the SZ, 1 gene for the KH, and 1 gene for CS, and several candidate genes for sesquiterpenoid biosynthesis were discovered based on the co-expression network. This study laid a solid foundation for further research on the sesquiterpenoid biosynthesis pathway, analysis of the regulation mechanism, and mechanism for the accumulation of sesquiterpenoids in A. lancea.
