Effect of Rehmanniae Radix on depression-like behavior and hippocampal monoamine neurotransmitters of chronic unpredictable mild stress model rats.
10.19540/j.cnki.cjcmm.20220421.401
- Author:
Ping TIAN
1
;
Wei ZHANG
1
;
Kai-Yan LI
1
;
Hong-Wei LI
2
;
Kai MA
1
;
De-En HAN
2
Author Information
1. Henan Academy of Chinese Medicine Zhengzhou 450004, China.
2. School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.
- Publication Type:Journal Article
- Keywords:
Rehmanniae Radix;
chronic unpredictable mild stress(CUMS);
depression;
hippocampus;
monoamine neurotransmitters
- MeSH:
3,4-Dihydroxyphenylacetic Acid/pharmacology*;
Animals;
Antidepressive Agents/therapeutic use*;
Chromatography, Liquid;
Depression/drug therapy*;
Disease Models, Animal;
Dopamine;
Eosine Yellowish-(YS)/pharmacology*;
Hematoxylin/pharmacology*;
Hippocampus/metabolism*;
Homovanillic Acid/pharmacology*;
Hydroxyindoleacetic Acid/metabolism*;
Methoxyhydroxyphenylglycol/pharmacology*;
Monoamine Oxidase/metabolism*;
Neurotransmitter Agents/metabolism*;
Norepinephrine/pharmacology*;
Plant Extracts;
Rats;
Rehmannia/chemistry*;
Serotonin/metabolism*;
Serotonin Plasma Membrane Transport Proteins/pharmacology*;
Stress, Psychological/metabolism*;
Tandem Mass Spectrometry;
Tryptophan Hydroxylase/metabolism*
- From:
China Journal of Chinese Materia Medica
2022;47(17):4691-4697
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of Rehmanniae Radix on depression-like behavior and monoamine neurotransmitters of chronic unpredictable mild stress(CUMS) model rats. CUMS combined with isolated feeding was used to induce the depression model of rats. The depression-like behavior of rats was evaluated by sucrose preference test, open field test, and forced swim test. Hematoxylin-Eosin(HE) staining was used to investigate the pathological changes of neurons in the CA1 and CA3 area of hippocampus. Ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS) was used to detect the contents of 5-hydroxytryptamine(5-HT), 5-hydroxyindoleacetic acid(5-HIAA), dopamine(DA), 3,4-dihydroxyphenylacetic acid(DOPAC), homovanillic acid(HVA), norepinephrine(NE), and 3-methoxy-4-hydroxyphenyl glycol(MHPG) in rats. Western blot was used to detect the protein expressions of tryptophan hydroxylase 2(TPH2), serotonin transporter(SERT), and monoamine oxidase A(MAO-A) in the hippocampus of rats. Compared with the normal group, depressive-like behavior of rats was obvious in the model group. The arrangements of neurons in the CA1 and CA3 area of hippocampus were loose and disorderly. The levels of 5-HT, 5-HIAA, and 5-HT/5-HIAA in the hippocampal area were decreased(P<0.01). The protein expression of TPH2 was decreased(P<0.01), but those of SERT and MAO-A were increased(P<0.01). In the Rehmanniae Radix groups with 1.8 g·kg~(-1) and 7.2 g·kg~(-1), the depression-like behavior of CUMS rats and pathological changes of neurons in CA1, CA3 area of hippocampus were improved. The protein expression of TPH2(P<0.05, P<0.01) was increased, and those of SERT and MAO-A were down-regulated(P<0.05, P<0.01). The levels of 5-HT, 5-HIAA, and 5-HT/5-HIAA in hippocampus were increased(P<0.05, P<0.01). The changes in DA, DOPAC, HVA, DA/(DOPAC +HVA), NE, DHPG, and NE/DHPG were not statistically significant. The results suggested that Rehmanniae Radix improved depression-like behavior of CUMS rats, and the mechanism might be related to the regulation of synthesis, transportation, and metabolism of 5-HT neurotransmitter in the hippocampus.
