Study on drug properties of Arisaematis Rhizoma and Arisaema Cum Bile based on substance and energy metabolism in normal and cold/heat syndrome model rats.
10.19540/j.cnki.cjcmm.20220524.402
- Author:
Fa-Zhi SU
1
;
Chen-Xi BAI
1
;
Wen-Sen ZHANG
1
;
Jing YANG
1
;
Jian-Ping HU
1
;
Yan-Ping SUN
1
;
Bing-You YANG
1
;
Hai-Xue KUANG
1
;
Qiu-Hong WANG
2
Author Information
1. Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine Harbin 150040, China.
2. Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine Harbin 150040, China School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
Arisaema Cum Bile;
Arisaematis Rhizoma;
cold and heat drug property;
energy metabolism;
substance metabolism
- MeSH:
AMP-Activated Protein Kinases;
Animals;
Arisaema/chemistry*;
Bile;
Chloral Hydrate;
Cold-Shock Response/drug effects*;
Drugs, Chinese Herbal/therapeutic use*;
Energy Metabolism;
Heat Stroke/therapy*;
Hot Temperature;
Male;
Rats;
Rats, Sprague-Dawley;
Saline Solution;
Syndrome;
TOR Serine-Threonine Kinases;
Thyroxine;
Water
- From:
China Journal of Chinese Materia Medica
2022;47(17):4682-4690
- CountryChina
- Language:Chinese
-
Abstract:
This paper clarified the scientific connotation of the changes in cold and heat properties of Arisaematis Rhizoma and Arisaema Cum Bile through investigating the changes of substance and energy metabolism after drug intervention in the rats with normal and cold/heat syndrome, so as to improve the method of evaluating the drug properties of Chinese medicine. After one week of adaptive feeding, healthy male SD rats were randomly divided into three parts: normal rats, heat syndrome rat models, and cold syndrome rat models. Through ice water bath and oral euthyrox(120 μg·kg~(-1)), the models of cold syndrome and heat syndrome were induced, respectively. The models were made at 9:00 am. and administrated by gavage at 3:00 pm. every day. All administration groups were administrated with Arisaematis Rhizoma and Arisaema Cum Bile decoction, respectively, and the blank group was given the same dose of normal saline. After continuous administration for 15 d, the rats were anesthetized by chloral hydrate, blood was taken from abdominal aorta, and the hearts and livers were removed and stored at-80 ℃. The changes in the body weight and anal temperature of rats during administration were detected, and the liver coefficient of rats was detected after removing the liver. Enzyme-linked immunosorbent assay(ELISA) was adopted to detect the expression level of the indexes related to substance and energy metabolism in liver and heart of rat, and Western blot was used to detect the expression of key proteins in AMPK/mTOR signaling pathway for further verification. The results showed that Arisaematis Rhizoma enhanced the expression level of enzymes related to substance and energy metabolism in the normal and cold and heat syndrome rat models, and increased anal temperature, which exhibited warm(hot) drug property. Arisaema Cum Bile inhibited the level of substance and energy metabolism in rats, and reduced anal temperature, which showed cold(cool) drug property. Chinese Pharmacopoeia has recorded "Arisaematis Rhizoma has warm property and Arisaema Cum Bile has cool property", which is consistent with the phenomenon in this study. Therefore, it is feasible to evaluate the drug properties of Chinese medicine based on the substance and energy metabolism of normal and cold/heat syndrome model rats, which completes the method of evaluating drug properties of Chinese medicine.
