Mechanism of Shaofu Zhuyu Decoction in treatment of endometriosis-associated dysmenorrhea with syndrome of cold coagulation and blood stasis based on MSK1/2.
10.19540/j.cnki.cjcmm.20220121.401
- Author:
Yuan-Huan CHEN
1
;
Hai-Yan MAO
1
;
Quan-Sheng WU
1
;
Xiao-Hua ZHANG
1
;
Jian SHEN
1
;
Peng FENG
1
;
Can-Can HUANG
1
;
Xiu-Jia JI
1
Author Information
1. Clinical College of Chinese Medicine, Gansu University of Chinese Medicine Lanzhou 730000, China.
- Publication Type:Journal Article
- Keywords:
DUSP1;
IL-10;
IL-6;
MSK1;
MSK2;
PGE2;
Shaofu Zhuyu Decoction;
TNF-α;
dysmenorrhea;
endometriosis;
syndrome of cold coagulation and blood stasis
- MeSH:
Animals;
Female;
Rats;
Anti-Inflammatory Agents/therapeutic use*;
Cytokines;
Dinoprostone;
Drugs, Chinese Herbal/therapeutic use*;
Dual-Specificity Phosphatases;
Dysmenorrhea/genetics*;
Endometriosis/genetics*;
Gestrinone/therapeutic use*;
Interleukin-10;
Interleukin-6;
Mitogen-Activated Protein Kinase 8/therapeutic use*;
Mitogens/therapeutic use*;
RNA, Messenger;
Tumor Necrosis Factor-alpha/metabolism*
- From:
China Journal of Chinese Materia Medica
2022;47(17):4674-4681
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to decipher the mechanism underlying the effect of Shaofu Zhuyu Decoction on endometriosis(EMT)-associated dysmenorrhea in rats with the syndrome of cold coagulation and blood stasis based on mitogen-and stress-activated protein kinase 1/2(MSK1/2).We employed a random number table to randomly assign SPF female non-pregnant rats into the sham group, and treated the rest rats with autologous transplantation+refrigerator freezing for the modeling of the syndrome of cold coagulation and blood stasis.The modeled rats were then randomly assigned into the control group and high-, medium-and low-dose Shaofu Zhuyu Decoction groups.The rats in the low-, medium-, and high-dose decoction groups were respectively administrated with 9, 4.5, and 2.3 g·kg~(-1) decoction through gavage once a day for 2 consecutive weeks, and those in the control group were administrated with 0.24 mg·kg~(-1) gestrinone through gavage once every 3 days for 2 weeks.After that, the size of ectopic focus in each rat was measured via laparotomy.Enzyme-linked immunosorbent assay(ELISA) was adopted to determine the expression of interleukin(IL)-6, IL-10, prostaglandin E2(PGE2), tumor necrosis factor-α(TNF-α).Western blot was employed to determine the protein levels of MSK1/2 and dual-specificity phosphatase 1(DUSP1) and real-time quantitative polymerase chain reaction(RT-PCR) to determine the mRNA levels of the two genes in rat eutopic endometrial tissue.Compared with the sham group, the model group showed increased levels of IL-6, PGE2, and TNF-α while decrease level of IL-10 in the serum(P<0.01).Compared with the model group, the high-and medium-dose decoction groups and the gestrinone group had declined levels of IL-6, PGE2, and TNF-α while risen level of IL-10 in the serum(P<0.01).The model group had lower protein levels and mRNA levels of MSK1/2 and DUSP1 in the eutopic endometrial tissue than the sham group(P<0.01). The high-and medium-dose decoction groups and the gestrinone group had higher protein and mRNA levels of MSK1/2 and DUSP1 in the eutopic endometrial tissue than the model group(P<0.01).The results indicated that Shaofu Zhuyu Decoction can regulate the abnormal expression of pro-inflammatory cytokines TNF-α, IL-6, and PGE2 and anti-inflammatory cytokines IL-10 and DUSP1 via MSK1/2 to alleviate EMT-associated dysmenorrhea in rats with the syndrome of cold coagulation and blood stasis.
