Chemical constituents from Artocarpus incisus and their inhibitory effects on proliferation of synoviocytes in vitro.
10.19540/j.cnki.cjcmm.20220506.203
- Author:
Yu-Tong XIE
1
;
Shu-Hong XIONG
1
;
Yuan BIAN
1
;
Yu WANG
1
;
Ruo-Qing GUAN
1
;
Xin-Yuan SUO
1
;
Meng-Ran DU
1
;
Yan-Ping LIU
2
;
Yan-Hui FU
2
Author Information
1. Key Laboratory of Tropical Medicinal Resources Chemistry of Ministry of Education, Hainan Normal University Haikou 571158, China.
2. Key Laboratory of Tropical Medicinal Resources Chemistry of Ministry of Education, Hainan Normal University Haikou 571158, China Hainan Provincial Engineering Research Center of Key Technology for Industrialization of Southern Medicinal Plant Resources, Hainan Normal University Haikou 571158, China Haikou Key Laboratory of Tropical Fruit and Vegetable Research and Development, Hainan Normal University Haikou 571158, China.
- Publication Type:Journal Article
- Keywords:
A.incisus;
Artocarpus;
anti-rheumatoid arthritis activities;
flavonoid derivatives;
sesquiterpenes
- MeSH:
Arthritis;
Artocarpus;
Cell Proliferation;
Ethanol;
Genistein;
Plant Extracts/pharmacology*;
Silica Gel;
Synoviocytes
- From:
China Journal of Chinese Materia Medica
2022;47(17):4665-4673
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents from the branches and leaves of Artocarpus incisus were isolated and purified via silica gel, ODS, and Sephadex LH-20 column chromatography as well as preparative HPLC. The chemical structures of all isolated compounds were identified in the light of their physicochemical properties, spectroscopic analyses, and comparisons of their physicochemical and spectroscopic data with the reported data in literature. As a result, 20 compounds were isolated and characterized from the 90% ethanol extract of the branches and leaves of A. incisus, which were identified as tephrosin(1), 6-hydroxy-6 a, 12 a-dehydrodeguelin(2), sarcolobin(3), lupiwighteone(4), 12-deoxo-12α-methoxyelliptone(5), 6 aα,12 aα-12 a-hydroxyelliptone(6), homopterocarpin(7), 3-hydroxy-8,9-dimethoxypterocarpan(8), pterocarpin(9), maackiain(10), medicarpin(11), calycosin(12), genistein(13), formononetin(14), 5-hydroxy-4',7-dimethoxy isoflavone(15), liquiritigenin(16), 4(15)-eudesmene-1β,7α-diol(17), ent-4(15)-eudesmene-1β,6α-diol(18), 1α-hydroxyisodauc-4-en-15-al(19), and guaianediol(20). Except compounds 13 and 16, all other compounds were isolated from the Artocarpus plants for the first time. Additionally, using MTS assay, compounds 1-20 were eva-luated for their anti-rheumatoid arthritis activities by measuring their anti-proliferative effects on synoviocytes in vitro. As a consequence, compounds 1-16 showed notable anti-rheumatoid arthritis activities, which displayed inhibitory effects on the proliferation of MH7 A synovial fibroblast cells, with the IC_(50) values in range of(9.86±0.09)-(218.07±1.96) μmol·L~(-1).
