Two new monoterpenoid indole alkaloids from hook-bearing branches of Uncaria sessilifructus.
10.19540/j.cnki.cjcmm.20220419.201
- Author:
Xiao YU
1
;
Lin-Lin ZHU
1
;
Chang XU
1
;
Cheng-Yuan SU
1
;
Yan LIANG
1
;
Bin LIU
1
;
Wei ZHANG
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
- Publication Type:Journal Article
- Keywords:
Uncaria sessilifructus;
alkaloid;
chemical constituent;
neuroprotective activity
- MeSH:
Animals;
Biological Products;
Drugs, Chinese Herbal/pharmacology*;
Ethanol;
Glucose;
Indole Alkaloids;
Neuroprotective Agents/pharmacology*;
Oxygen;
Rats;
Secologanin Tryptamine Alkaloids;
Silica Gel;
Uncaria/chemistry*
- From:
China Journal of Chinese Materia Medica
2022;47(17):4650-4657
- CountryChina
- Language:Chinese
-
Abstract:
The present study investigated the chemical constituents from Uncaria sessilifructus and their neuroprotective activities. The compounds were separated and purified from the 90% ethanol extract of U. sessilifructus by various chromatographic methods, including silica gel, Sephadex LH-20, and semi-preparative HPLC. Seven compounds were obtained, and their structures were identified as uncanidine J(1), uncanidine K(2), 17-O-ethylhirsutine(3), tetrahydroalstonine(4), akuammigine(5), hirsutine(6), and hirsuteine(7) by physicochemical properties and various spectral techniques, including UV, IR, MS, and NMR. Compounds 1 and 2 are two new compounds. Compound 3 is a new natural product, and compound 4 was isolated from U. sessilifructus for the first time. In addition, the isolated compounds were evaluated for their neuroprotective effects on oxygen and glucose deprivation/reoxygenation(OGD/R) injury in primary cortical neurons in rats. The results showed that compounds 1-7 had different degrees of protective effects on OGD/R injury. The EC_(50) values of compounds 2-4 were(0.17±0.03),(1.70±0.38), and(1.79±0.23) μmol·L~(-1), respectively.
