Genetic diversity and population structure of germplasm resources of Amomum villosum based on SSR markers.
10.19540/j.cnki.cjcmm.20220514.101
- Author:
Wen-Xiu LI
1
;
Jin-Liang LI
2
;
Jun-Jun HE
3
;
Hua-Lin ZHANG
1
;
Ping LUO
1
;
Ying WEI
4
;
Mei-Ting ZHAO
4
Author Information
1. Zhanjiang Experimental Station/Guangdong Dry Farming Water-saving Agricultural Engineering Technology Research Center, China Academy of Tropical Agricultural Sciences Zhanjiang 524091, China.
2. School of Life Sciences, Sun Yat-Sen University Guangzhou 510275, China.
3. Zhanjiang Experimental Station/Guangdong Dry Farming Water-saving Agricultural Engineering Technology Research Center, China Academy of Tropical Agricultural Sciences Zhanjiang 524091, China South Subtropical Crop Research Institute, China Academy of Tropical Agricultural Sciences Zhanjiang 524091, China.
4. College of Tropical Crops, Yunnan Agricultural University Simao 665099, China.
- Publication Type:Journal Article
- Keywords:
Amomum villosum;
SSR molecular marker;
cluster analysis;
genetic diversity
- MeSH:
Alleles;
Amomum/genetics*;
China;
Genetic Variation;
Microsatellite Repeats/genetics*;
Plant Breeding
- From:
China Journal of Chinese Materia Medica
2022;47(17):4618-4626
- CountryChina
- Language:Chinese
-
Abstract:
Amomum villosum, serving as an important medicinal material, is complex in the genetic background of germplasm resources. Exploring the genetic diversity and genetic relationship of germplasm resources is conducive to clarifying the germplasm source and genetic background of A. villosum, so as to improve the efficiency of parent selection and variety breeding of A. villosum. Seventy-one pairs of SSR primers were used for PCR amplification of 84 A. villosum samples by polyacrylamide gel electrophoresis. Fifty-four pairs of SSR primers with high polymorphism were screened out for the analysis of genetic diversity. The results showed that 293 alleles were detected from 84 germplasm resources by 54 pairs of SSR primers, with an average of 5.32 alleles for each pair of primers, and a variation range of 3-8, and the primer AVL12 marked the highest number of alleles. The PIC value of each locus varied from 0.068 7 to 0.828 9, with an average of 0.529 9, and the highest was marked by AVL24. The genetic diversity of A. villosum was the highest in Yunnan, followed by Guangxi, and the lowest was found in Guangdong. The population structure analysis and cluster analysis showed that the samples were classified into two groups. In terms of origin, samples from Yunnan and Guangxi had a close genetic relationship, and there was no obvious differentiation of A, villosum resources from different origins. In this study, 54 pairs of SSR markers were used to analyze the genetic diversity and population structure of 84 germplasm resources, which can reflect the genetic relationship between A. villosum samples from different germplasm sources and different populations, thus providing a theoretical basis for the collection, research, and breeding of A. villosum resources.
