Comparison between macroscopic identification and DNA barcoding identification of Amomi Fructus.
10.19540/j.cnki.cjcmm.20220515.101
- Author:
En-Ai ZHAI
1
;
Wen-Juan MI
2
;
Yang CUI
1
;
Wei-Feng HONG
1
;
Ya-Shun WANG
1
;
Xing-Yu GUO
1
;
Hui-Qin ZOU
1
;
Yong-Hong YAN
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
2. Jiangsu Simcere Pharmaceutical Co., Ltd. Nanjing 210042, China.
- Publication Type:Journal Article
- Keywords:
100-fruit weight;
Amomi Fructus;
DNA barcoding;
electronic nose;
macroscopic identification
- MeSH:
Camphanes;
Camphor/analysis*;
DNA Barcoding, Taxonomic;
Drugs, Chinese Herbal/chemistry*;
Fruit/genetics*;
Limonene/analysis*;
Reproducibility of Results
- From:
China Journal of Chinese Materia Medica
2022;47(17):4600-4608
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to explore the consistency between macroscopic identification and DNA barcoding identification of Amomi Fructus. With the DNA barcoding identification results, we evaluated the reliability of identifying Amomi Fructus quality by combining macroscopic traits with main volatile chemical components. Thirteen batches of Amomi Fructus samples were collected for identification. Firstly, the morphological and sensory characteristics of each sample were observed and recorded according to the standard in Chinese Pharmacopoeia(2020 edition). The 100-fruit weight, longitudinal diameter, transverse diameter, and longitudinal diameter-to-transverse diameter ratio were measured, which correspond to large, solid, and full kernel representing good quality in the sensory evaluation. The odor value detected by electronic nose and major volatile components(borneol, camphor, limonene, and borneol acetate) correspond to the sensory evaluation of strong odor representing good quality. Secondly, DNA barcoding was employed to identify the 13 batches of samples. Finally, clustering analysis was performed for the main volatile components and macroscopic traits, and the identification results were compared with those of DNA barcoding. Except two batches of samples(No.6 and No.10), the macroscopic identification showed the results consistent with those of DNA barcoding, with an identification rate of 84.62%. The clustering results of the content of four volatile chemical components and macroscopic traits were also consistent with the DNA barcoding identification results. DNA barcoding can verify the results of macroscopic identification and provide a scientific basis for the inheritance and development of macroscopic identification. Moreover, the combination of macroscopic traits and chemical components demonstrates higher accuracy in the quality evaluation of Chinese medicinal materials.
