Toxicokinetics of emodin-8-O-β-D-glucoside in rats in vivo.
10.19540/j.cnki.cjcmm.20220516.701
- Author:
Qi WANG
1
;
Jian-Bo YANG
1
;
Ying WANG
1
;
Yan-Yi LI
2
;
Hai-Ruo WEN
1
;
Yu-Jie ZHANG
3
;
Shuang-Cheng MA
1
Author Information
1. National Institutes for Food and Drug Control Beijing 100050, China.
2. National Institutes for Food and Drug Control Beijing 100050, China Beijing University of Chinese Medicine Beijing 100029, China.
3. Beijing University of Chinese Medicine Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
emodin-8-O-β-D-glucoside;
metabolites;
toxicokinetics
- MeSH:
Animals;
Anthraquinones;
Chromatography, High Pressure Liquid/methods*;
Emodin/toxicity*;
Glucosides/toxicity*;
Mass Spectrometry;
Rats;
Toxicokinetics
- From:
China Journal of Chinese Materia Medica
2022;47(15):4214-4220
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to establish an ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) method for the determination of emodin-8-O-β-D-glucoside(EG) and its metabolites in plasma, and to investigate the toxicokinetics(TK) behavior of them in rats. To be specific, the TK of EG and its metabolites from the first to the last administration in the repeated dose toxicity study was determined, and the kinetic parameters were calculated. The exposure of EG prototype and metabolites in rat plasma after oral administration of different doses of EG was evaluated. The result showed that the prototype of EG and its metabolites aloe-emodin-8-O-β-D-glucoside, emodin, aloe-emodin, and hydroxyemodin could be detected in rats after oral administration of high-, medium-, and low-dose EG. The area under the curve(AUC) of the prototype and metabolites after the first and last administration was in positive correlation with the dose. The time to the maximum concentration(T_(max)) of EG and metabolites in the three administration groups was <6 h, and the longest in vivo residence time was 12 h. The T_(max) and in vivo residence time of EG were prolonged with the increase in the dose. The metabolites emodin, aloe-emodin, and hydroxyemodin all had two peaks. Both hydroxyemodin and aloe-emodin exhibited increased plasma exposure, slow metabolism, and accumulation in vivo. In addition, aloe-emodin-8-O-β-D-glucoside and emodin disappeared with the increase in dose, suggesting the change of the metabolic pathway of EG in vivo in the case of high-dose administration. The mechanism of high-dose EG in vivo needs to be further explored. This study preliminarily elucidates the TK behavior of EG in rats, which is expected to support clinical drug use.
