Identification of metabolites of imperatorin in rats: based on UHPLC-Q-Exactive Orbitrap MS.
10.19540/j.cnki.cjcmm.20220307.203
- Author:
Yi-Fang CUI
1
;
Hong WANG
2
;
Jian RUAN
3
;
Wen-Wen ZHANG
4
;
Hong-Yan ZHOU
2
;
Ya-Nan LI
2
;
Long DAI
1
;
Jia-Yu ZHANG
1
Author Information
1. College of Pharmacy, Shandong University of Traditional Chinese Medicine Ji'nan 250300, China School of Pharmacy, Binzhou Medical University Yantai 264003, China.
2. College of Pharmacy, Shandong University of Traditional Chinese Medicine Ji'nan 250300, China.
3. Yantai Testing Center for Food and Drug Yantai 264003, China.
4. School of Pharmacy, Binzhou Medical University Yantai 264003, China.
- Publication Type:Journal Article
- Keywords:
UHPLC-Q-Exactive Orbitrap MS;
imperatorin;
metabolites in vivo
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Feces;
Furocoumarins;
Plasma;
Rats;
Solid Phase Extraction
- From:
China Journal of Chinese Materia Medica
2022;47(15):4202-4213
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to identify and analyze the metabolites of imperatorin in rats by UHPLC-Q-Exactive Orbitrap MS. Specifically, after rats were treated(ig) with imperatorin, the plasma, urine, and feces were collected, and the samples were processed by solid phase extraction. Then, UHPLC-Q-Exactive Orbitrap MS was performed. In MS, 0.1% formic acid water(A)-acetonitrile(B) was applied as mobile phase for gradient elution and the data of MS in both positive and negative ion modes were collected. The metabolites of imperatorin in blood, urine, and feces of rats were analyzed to explore the metabolic pathways of imperatorin in rats. According to accurate molecular weight, multistage MS data, MS fragmentation rule of the standard substance, and previous reports, a total of 51 metabolites were identified, with 35, 40, and 16 from plasma, urine, and feces, separately. The main metabolic pathways were oxidization, glucuronidation, isopentenyl removal, sulphation, carboxylation, among others. The conclusion in this study is expected to serve as a reference for the further development and the further pharmacodynamics study of imperatorin.
