Effect of modified Danggui Shaoyao Powder on SOCS3/TLR4 signaling pathway in rats with chronic atrophic gastritis.
10.19540/j.cnki.cjcmm.20220325.701
- Author:
Xiao-Jia ZHENG
1
;
Ping-Ping CHEN
1
;
Yang LIU
1
;
Jian-Hui SUN
1
;
Nai-Lin ZHANG
1
;
Bin WANG
1
;
Qi-Quan LIU
2
Author Information
1. Hebei University of Chinese Medicine Shijiazhuang 050020, China.
2. Hebei University of Chinese Medicine Shijiazhuang 050020, China the First Affiliated Hospital of Hebei University of Chinese Medicine Shijiazhuang 050011, China.
- Publication Type:Journal Article
- Keywords:
chronic atrophic gastritis;
modified Danggui Shaoyao Powder;
suppressor of cytokine signaling 3/Toll-like receptor 4 signaling pathway
- MeSH:
Animals;
Atrophy;
Gastritis, Atrophic/genetics*;
Interleukin-6/metabolism*;
Myeloid Differentiation Factor 88/metabolism*;
NF-kappa B/metabolism*;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
Powders;
RNA, Messenger;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
Suppressor of Cytokine Signaling 3 Protein/metabolism*;
Toll-Like Receptor 4/metabolism*;
Tumor Necrosis Factor-alpha/metabolism*;
bcl-2-Associated X Protein/metabolism*
- From:
China Journal of Chinese Materia Medica
2022;47(15):4128-4135
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the effect of modified Danggui Shaoyao Powder on the suppressor of cytokine signaling 3(SOCS3)/Toll-like receptor 4(TLR4) signaling pathway in gastric tissue of rats with chronic atrophic gastritis(CAG).Sixty SPF-grade SD rats were randomly assigned into the normal group, model group, Moluo Pills group, and high-, medium-, and low-dose groups of modified Danggui Shaoyao Powder.The rats in other groups except the normal group were treated with N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) to establish the CAG model.After 12 weeks of modeling, the rats in each group were administrated with corresponding drugs by gavage for 8 weeks.After the last administration, the histopathological changes of rat gastric mucosa were observed via hematoxylin-eosin(HE) staining.The serum levels of IL-6, TNF-α, and CRP were determined by enzyme-linked immunosorbent assay(ELISA).The mRNA levels of SOCS3 and TLR4 were determined by real-time PCR.The protein levels of SOCS3, TLR4, JAK2, p-JAK2, STAT3, and p-STAT3 in rat gastric tissue were measured by Western blot.Immunohistochemical method was employed to determine the protein levels of NF-κB, MyD88, NLRP3, Bcl-2, Bax, and Bad in rat gastric tissue.The results showed that modified Danggui Shaoyao Powder alleviated gastric mucosal atrophy of rats, significantly lowered the levels of IL-6, TNF-α, and CRP in rat serum, up-regulated the mRNA level of SOCS3, and down-regulated the mRNA level of TLR4 in rat gastric tissue.Furthermore, modified Danggui Shaoyao Powder up-regulated the protein level of SOCS3, down-regulated the protein levels of TLR4, p-JAK2, p-STAT3, NF-κB, MyD88, NLRP3, Bax, and Bad, and promoted the expression of Bcl-2 protein.Therefore, modified Danggui Shaoyao Powder may mitigate the gastric mucosal atrophy of rats by regulating the SOCS3/TLR4 signaling pathway.
