Construction and evaluation of a gradient stress model of PC12 cells induced by corticosterone.
10.12047/j.cjap.6286.2022.036
- Author:
Ming-Zhe LI
1
;
Long-Fei XU
1
;
Zhao-Li CHEN
1
;
Xin-Xing WANG
1
;
Ling-Ling PU
1
;
Wei-Li LIU
1
;
Tian-Hui WANG
1
Author Information
1. Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050.
- Publication Type:Journal Article
- Keywords:
PC12 cells;
cell culture;
cell model;
corticosterone;
stress
- MeSH:
Animals;
Cell Survival;
Corticosterone/pharmacology*;
NAD/pharmacology*;
PC12 Cells;
Rats;
Superoxide Dismutase
- From:
Chinese Journal of Applied Physiology
2022;38(3):284-288
- CountryChina
- Language:Chinese
-
Abstract:
Objective: A gradient stress model of PC12 cells induced by corticosterone was established to provide a basis for the evaluation and regulation of cell stress. Methods: The effect of corticosterone on cell viability was observed by measuring PC12 cell viability at different concentrations of corticosterone (0~1 000 μmol/L) after different intervention times (8~48 h) to screen the cell models for optimal intervention conditions. Key stress indicators (MDA, SOD, NADH, LDH) were measured spectrophotometrically and microscopically to evaluate the models. Results: When the concentration of corticosterone was below 200 μmol/L and the intervention time was 12 h, the cell viability was below half inactivation rate, which could reduce the confounding factors due to the decrease of cell viability in each group. Compared with the blank control group, corticosterone increased the levels of MDA, NADH and LDH,and decreased the levels of SOD in the model group in a concentration-dependent manner (P＜0.01), which was consistent with the construction of the gradient stress model. Conclusion: A gradient stress injury model of PC12 cells was successfully established, with intervention concentrations of 0 μmol/L, 25 μmol/L, 50 μmol/L, 100 μmol/L, 150 μmol/L and 200 μmol/L corticosterone at an intervention time of 12 h. The degree of stress injury of the cell model was increased gradually, which could be used as a basis and object for conducting cell stress injury assessment and regulation experiments.
