Quantitative proteomics reveal the potential biological functions of the deubiquitinating enzyme Ubp14 in <i>Saccharomyces cerevisiae</i>.

Zhaodi LI; Qiuyan Lan; Yanchang LI; Cong XU; Lei Chang; Ping XU; Changzhu Duan

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Quantitative proteomics reveal the potential biological functions of the deubiquitinating enzyme Ubp14 in Saccharomyces cerevisiae.

Author: Zhaodi LI ¹ ; Qiuyan LAN ² ; Yanchang LI ² ; Cong XU ² ; Lei CHANG ² ; Ping XU ² ; Changzhu DUAN ¹
Author Information

1. Molecular Medicine and Cancer Research Center, Department of Cell Biology and Genetics, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China.
2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Research Unit of Proteomics & Research and Development of New Drug, Chinese Academy of Medical Sciences, Beijing Institute of Lifeomics, Academy of Military Medical Sciences of Academy of Military Science, Beijing 102206, China.
Publication Type:Journal Article
Keywords: Ubp14; deubiquitinating enzyme; stable isotope labeling by amino acids in cell culture (SILAC); ubiquitin
MeSH: Saccharomyces cerevisiae/metabolism*; Proteomics; Endopeptidases/metabolism*; Ubiquitin/metabolism*; Ubiquitination; Proteins/metabolism*; Deubiquitinating Enzymes/metabolism*; Biological Phenomena
From: Chinese Journal of Biotechnology 2022;38(10):3901-3913
CountryChina
Language:Chinese
Abstract: Ubiquitination is one of the reversible protein post-translational modifications, in which ubiquitin molecules bind to the target protein in a cascade reaction of ubiquitin activating enzymes, ubiquitin conjugating enzymes, and ubiquitin ligases. The deubiquitinating enzymes (DUBs) remove ubiquitin residues from the substrates, which play key roles in the formation of mature ubiquitin, the removal and trimming of ubiquitin chains, as well as the recycling of free ubiquitin chains. Ubp14, a member of the ubiquitin specific proteases family in Saccharomyces cerevisiae, is mainly responsible for the recycling of intracellular free ubiquitin chains. To investigate its global biological function, a ubp14∆ mutant was constructed by homologous recombination technique. The growth rate of ubp14∆ mutant was lower than that of the wild-type (WT) strain. Using stable isotope labeling by amino acids in cell culture (SILAC) combined with deep coverage proteomics analysis, the differentially expressed proteins of ubp14∆ mutant relative to the wild-type strain were systematically analyzed. A total of 3 685 proteins were identified in this study, and 109 differentially expressed proteins were filtered out by statistical analysis. Gene ontology analysis found that differentially expressed proteins caused by Ubp14 loss were mainly involved in amino acid metabolism, REDOX, heat shock stress and etc, which shed light on the broad biological function of this DUB. This study provides highly reliable proteomic data for further exploring the biological functions of the deubiquitination enzyme Ubp14, and further understanding the relationship between the free ubiquitin homeostasis and biological process regulation.