Changes in the urine proteome in an acute hypoxic rat model.

Yijin Bao; Xiang Cheng; Lingling Zhu; Ming Fan; Youhe Gao

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Changes in the urine proteome in an acute hypoxic rat model.

Author: Yijin BAO ¹ ; Xiang CHENG ² ; Lingling ZHU ² ; Ming FAN ² ; Youhe GAO ¹
Author Information

1. Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing Normal University, Beijing 100875, China.
2. Institute of Military Cognition and Brain Sciences, Beijing 100850, China.
Publication Type:Journal Article
Keywords: animal model; hypoxia; proteomics; urine
MeSH: Animals; Rats; Proteome/analysis*; Chromatography, Liquid/methods*; Tandem Mass Spectrometry/methods*; Proteomics/methods*; Hypoxia
From: Chinese Journal of Biotechnology 2022;38(10):3878-3887
CountryChina
Language:Chinese
Abstract: The purpose of this study was to explore the effect of acute hypoxia on urine proteome in rats. In this study, rats were placed in a hypoxic chamber simulating a plateau environment at an altitude of 5 000 m for 24 hours. Urine samples were collected at 0, 12, and 24 h after hypoxia. Urinary proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with the control (before hypoxia), a total of 144 differentially expressed proteins were identified in the hypoxia 12 h group, and 129 differentially expressed proteins were identified in the hypoxia 24 h group. Functional annotation analysis revealed that these differentially expressed proteins were involved in a series of biological pathways related to hypoxic stress, such as anti-oxidative stress, glycolysis, complement and coagulation cascade. Our results suggest that the urinary proteome can reflect significant changes upon acute hypoxic stimulation. These findings may provide an approach to judge the hypoxia state of the body and help to assist the detection of hypoxia state.