Advances in the methods of phosphopeptide enrichment and separation in phosphoproteomic research.
- Author:
Jiaran LI
1
;
Xiulan CHEN
1
;
Fuquan YANG
1
Author Information
- Publication Type:Journal Article
- Keywords: phosphopeptide; phosphopeptide enrichment; phosphopeptide fractionation; phosphoproteomics
- MeSH: Phosphopeptides/metabolism*; Proteomics/methods*; Titanium/chemistry*; Chromatography, Affinity; Proteome; Phosphorylation
- From: Chinese Journal of Biotechnology 2022;38(10):3648-3658
- CountryChina
- Language:Chinese
- Abstract: The systematic and in-depth study of phosphoproteome rely on highly reproducible and specific phosphopeptide enrichment methods. At present, a variety of enrichment methods have been developed based on different principles, and these methods often display different selectivity and specificity. It is therefore very important to select the most suitable enrichment method according to different research purposes. This review summarized the phosphopeptide enrichment based on affinity chromatography, immunoprecipitation, chemical derivatization, chromatography and other newly developed methods. The advantages and disadvantages of these methods, as well as the related optimization and improvement strategies, were discussed in detail. In addition, we also briefly summarized the progress of the combination of phosphopeptide enrichment and fractionation methods developed in recent years.
