Advances in microbial transcriptomics techniques.

Author: Yuanyuan GUO ¹ ; Yaru SUN ¹ ; Heping ZHANG ¹
Author Information

1. Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia, China.
Publication Type:Journal Article
Keywords: microarray; microbes; sequencing techniques; transcriptomics
MeSH: Transcriptome; Oligonucleotide Array Sequence Analysis; Sequence Analysis; RNA; Biotechnology
From: Chinese Journal of Biotechnology 2022;38(10):3606-3615
CountryChina
Language:Chinese
Abstract: With the rapid development of molecular biotechnology, transcriptomics has been widely used in the study of gene expression. In recent years, the techniques for microbial transcriptomics research have also been rapidly developing. At the gene level, the way for obtaining sequence information has been developed from complementary validation of RNA fragment through DNA microarray to direct sequencing of full-length RNA. Spatially, the traditional population transcriptomics technique has been developed into spatial, single cell and epigenetic transcriptomics studies. With the application of transcriptomics techniques in the field of microbial research, the corresponding defects were gradually revealed and constantly improved. In this paper, the traditional and new transcriptomics techniques in the field of microbial research are summarized to provide reference for microbial transcriptomics research.