Effect of <i>eIF4B</i> knockout on apoptosis of mouse fetal liver cells.

Guoqing Wang; Biao Chen; Yuhai Chen; Qianwen Zhu; Min Peng; Guijie Guo; Jilong Chen

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Effect of eIF4B knockout on apoptosis of mouse fetal liver cells.

Author: Guoqing WANG ¹ ; Biao CHEN ² ; Yuhai CHEN ² ; Qianwen ZHU ¹ ; Min PENG ¹ ; Guijie GUO ¹ ; Jilong CHEN ¹
Author Information

1. Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
2. Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Publication Type:Journal Article
Keywords: cleaved-caspase 3; eIF4B; fetal liver; mTOR
MeSH: Animals; Apoptosis/genetics*; Caspase 3; Eosine Yellowish-(YS); Eukaryotic Initiation Factors/metabolism*; Fibroblasts; Hematoxylin; Liver/metabolism*; Mice; Ribosomal Protein S6 Kinases, 70-kDa/genetics*; TOR Serine-Threonine Kinases
From: Chinese Journal of Biotechnology 2022;38(9):3489-3500
CountryChina
Language:Chinese
Abstract: Eukaryotic translation initiation factor 4B (eIF4B) plays an important role in mRNA translation initiation, cell survival and proliferation in vitro, but the in vivo function is poorly understood. In this study, via various experimental techniques such as hematoxylin-eosin (HE) staining, flow cytometry, Western blotting, and immunohistochemistry, we investigated the role of eIF4B in mouse embryo development using an eIF4B knockout (KO) mouse model and explored the mechanism. We found that the livers, but not lungs, brain, stomach, or pancreas, derived from eIF4B KO mouse embryos displayed severe pathological changes characterized by enhanced apoptosis and necrosis. Accordingly, high expression of cleaved-caspase 3, and excessive activation of mTOR signaling as evidenced by increased expression and phosphorylation of p70S6K and enhanced phosphorylation of 4EBP1, were observed in mouse embryonic fibroblasts and fetal livers from eIF4B KO mice. These results uncover a critical role of eIF4B in mouse embryo development and provide important insights into the biological functions of eIF4B in vivo.